PLANT PHYSIOLOGY® ONLINE -- Watson et al. 131 (3): 1104 Table I

Table I. Proteins identified in barrel medic tissues
Table I contains a list of identified proteins from specific tissues of Medicago truncatula. The data are separated by tissue and include: an assigned protein spot no. (see Fig. 1), database accession no. of the best match, databases that yielded concurrent identifications, and the number of MALDI-TOFMS peptides matched. LC/MS/MS was performed on select proteins, and Mascot scores for these proteins are provided in parentheses. Not applicable (NA) denotes that no MALDI data was used in the identification. Significantly more detailed data supporting the protein identifications can be found in Supplemental Table I. Accession no. is GenBank no. Databases have following notations: N, NCBI; S, SwissProt; E, pdbESTothers; (E), MtESTonly. Species are noted as Hv, Hordeum vulgare; Sb, Sorghum bicolor, LE, Lycopersicon esculentum; and Mt, Medicago truncatula.

Tissue Spot # Identification Accession Number Databases # Peptides/LC/MS/MS

lvs 35 er ATPase (CDC48-like protein)^a NP190891 N 11

lvs 39 DNA mismatch repair protein^a O66652 S 8

lvs 47 Rubisco BE420942 E/Hv, (E) 7

lvs 52 Rubisco BE420942 E/Hv 5

lvs 51 F23N19.10, TPR repeat protein AW694998 E/Mt, (E) 4/(513)

lvs 63 Cell division prt. FTSK homolog^a P45264 S, N 8

lvs 78 Rubisco BAA20039 N, S, E/Hv 11

lvs 82 Rubisco AAF97663 N, S, E/Hv 9

lvs 84 Rubisco AAF15326 N, S, E/Hv 9

lvs 92 Rubisco X69528 N NA/(389)

lvs 98 Transcription factor^a BF004459 (E) 5

lvs 105 S-adenosyl-Met synthetase BG581653 E/Mt, N, S 12

lvs 108 S-adenosyl-Met synthetase P50303 S 8

lvs 111 Rubisco activase AF251264 N NA/(372)

lvs 113^b ATP synthase beta chain NP077960 N 8

lvs 113^b Rubisco activase Q42450 S, E/Sb 6

lvs 124 Rubisco activase AAG61120 N, S 9

lvs 126 Rubisco activase AAG61120 N, S 8

lvs 123 Aminomethyl transferase, mito. Precursor^a BF521422 E/Mt, (E) 12

lvs 128 Aminomethyl transferase, (T protein)^a P49364 N, S, E/Mt 11

lvs 136 Fru biphosphate aldolase BI309468 (E), N, S, E/Mt 9

lvs 138 Spermine synthase BE204391 E/Mt 5

lvs 139 Putative Arabidopsis thaliana protein^a AW685607 E/Mt 5

lvs 141 Ankyrin repeat protein AL388433 E/Mt 5

lvs 144^b Glyceraldehyde-3-phosphate dehydrogenase BF003409 E/Mt, (E) 10

lvs 144^b Possible tartrate dehydrogenase P70792 S, N 6

lvs 149^b Glyceraldehyde-3-phosphate dehydrogenase BG453922 (E) 7

lvs 149^b Tartrate dehydrogenase P70792 N, S 6

lvs 155 Leu2 (3-isopropyl malate dehydrogenase)^a P18120 N, S 7

lvs 158 Malate dehydrogenase T09286 N, E/Mt, (E) 14

lvs 196^b Ascorbate peroxidase BG587041 (E) 5

lvs 187 Oxygen evolving enhancer protein P14226 S, N, E/Mt, (E) 7

lvs 191 Oxygen evolving enhancer protein P14226 S, N, E/Mt, (E) 9

lvs 188 Remorin^a BG588209 (E) 7

lvs 189 Remorin^a BG588209 (E) 8

lvs 196^b Rubisco AAC35045 N, S 8

lvs 206^b Mitotic cyclin B1-1^a AAC24244 N 8

lvs 206^b ATP synthase^a BG582863 (E) 9

lvs 205 Oxygen-evolving enhancer protein 1 BG449793 (E) 5

lvs 219 Cystathione-B-lyase^a P53780 S 6

lvs 222 Chloro membrane-associated 30-kD protein/transit pept^a AW776774 E/Mt, (E) 9

lvs 223 RNA-binding protein BF641320 E/Mt, (E) 8

lvs 238 L-ascorbate peroxidase P48534 N, S, E/Mt, (E) 6

lvs 241 Ascorbate peroxidase AAL15164 N, S, (E) 6

lvs 237 Acid phosphatase BG588612 (E) 8

lvs 239 Acid phosphatase BG588612 (E) 11

lvs 251 Triose phosphate isomerase, cytosolic BF642390 E/Mt, (E) 10

lvs 250 ABC transporter^a NP488322 N 7

lvs 258^b Pyrimidine-nucleoside phosphorylase^a P39N9 S 8

lvs 263 Chaperonin 21 precursor^a AW775755 E/Mt 6

lvs 265 Chaperonin 21 precursor^a AW776607 E/Mt, (E) 5

lvs 261 Patatin-like protein^a AAF98369 N 5

lvs 258^b Transcription factor VSE-1^a CAA05898 N 6

lvs 270 Oxygen-evolving enhancer protein P16059 S, E/Mt, (E) 7

lvs 280 Oxygen-evolving enhancer protein BF521386 E/Mt, S, (E) 12

lvs 281 Plastid specific ribosomal protein^a BE318731 (E) 4

lvs 287 Gly-rich cell wall structural protein 2^a AL366848 E/Mt, (E) 8

lvs 284 Oxygen-evolving enhancer protein P16059 S, E/Mt, (E) 5

lvs 338 Hypothetical protein^a NP180029 N 5

lvs 363 Aspartate 1-decarboxylase precursor^a P52999 S 4

lvs 388 Rubisco small subunit BF520627 E/Mt, (E) 9

lvs 387 Rubisco small subunit BF519126 E/Mt 5

lvs 397 Plastocyanine precursor AW776926 E/M. t., (E) 7

lvs 422 Photosystem I iron-sulfur protein^a NP039445 N, S 8

stm 5 Cell division (valosin-containing) protein P54774 N, S 8

stm 7 Heat shock protein 70 1909352A N, S 10

stm 10 TPR repeat protein AW694998 (E), E/Mt 7

stm 9 Heat shock protein 70 P37900 N, S, E/Mt 8

stm 17 Rubisco CAA93074 N, S 8

stm 16 Rubisco P28400 N, S 7

stm 18^b ATP synthase^a CAB85681 N 7

stm 18^b Rubisco P30401 S, N 7

stm 19 Rubisco P04991 S, N, E/Mt 13

stm 20 Rubisco AAF97641 N, S, E/Mt 6

stm 22 Tubulin alpha chain Q43473 N, S, E/Mt 13

stm 23 26S proteasome AAA-ATPase subunit^a BE325937 E/Mt 5

stm 24 26S proteasome (TAT binding)^a NP187204 N, S, E/Mt 7

stm 26 SAM synthetase P46611 N, S, E/Mt 7

stm 29 Actin Q96483 N, S, E/Mt 7

stm 36 ATPase or P loop kinase^a NP347611 N 9

stm 37 Fru 1,6 biphosphate aldolase O65735 N, S, E/Mt 8

stm 39 Adenosine kinase^a BF004017 (E) 10

stm 40 Malate dehydrogenase BI310064 (E) 6

stm 42 Annexin^a T09552 N, E/Mt 6

stm 43 Fructokinase AW584645 E/Mt, (E) 7

stm 44 Ribose-phosphate pyrophosphokinase^a P47304 S 7

stm 45 IFR-like oxidoreductase BF644624 E/Mt, (E) 5

stm 48 Atran bp1a (Ran-binding protein 1 domain)^a AW686211 (E) 7

stm 46 Cinnamoyl-CoA reductase^a BF635045 (E) 7

stm 49 G protein beta subunit^a Q39836 N, S, E/Mt, (E) 4/(265)

stm 51 Oxygen-evolving enhancer protein I P14226 S, N 10

stm 52 RNA-binding protein-like NP196048 N 6

stm 57 Ascorbate peroxidase BG648814 (E) 4

stm 58 Proteasome subunit alpha type 7 (20S) Q9SXU1 S, N, E/Mt, (E) 5

stm 55 SAM:trans-caffeoyl CoA 3-O methyl transf.^a T09399 N, E/Mt 10

stm 54 RNA-binding protein-like BF641320 E/Mt 5

stm 60 Ascorbate peroxidase BG648814 (E), E/Mt 5

stm 61 Acid phosphatase BG588612 (E), E/Mt 6

stm 62 Triosphosphate isomerase BF642390 E/Mt, (E) 10

stm 64 Expressed protein^a AI774799 E/Le 5

stm 66 Uridylate monophosphate kinase AW981222 E/Mt, (E) 8

stm 70 23-kD O₂-evolving pht. sys. II precursor^a P16059 N, S, E/Mt, (E) 7

stm 72 ATP synthase, delta chain^a Q41000 S 4

stm 81 vcCYP AW775250 E/Mt, (E), N 8

stm 85 40S ribosomal protein S12^a AL375805 E/Mt 4

stm 86 Gly-rich RNA binding protein AL379229 E/Mt, (E) 4

stm 88 60S ribosomal protein AW776748 E/Mt, (E) 4

stm 90 Nucleoside diphosphate kinase I P47922 S 5

stm 89 Gly-rich RNA binding protein AA660717 E/Mt, (E), N 9

stm 95 Hypothetical^a NP174644 N 6

rts 2 Heat shock 70 Q02028 N, S, E/Mt 22

rts 5 Phosphoglyceromutase BG585916 (E) 5

rts 6 Protein disulfide isomerase BI309490 E/Mt, (E), N, S 10

rts 9 Putative methyl binding domain AL378817 E/Mt 4

rts 12 ATPase beta subunit CAA75477 N, S, E/Mt, (E) 13

rts 19 Actin isoform B T51183 N, S, E/Mt, (E) 13

rts 20 Peroxidase precursor AL369822 (E), E/Mt 8

rts 22 Ankyrin repeat protein HBP1 BI311773 (E), E/Mt 12

rts 28 Glyceraldehyde-3-phosphate dehydrogenase BG453922 (E), S 10

rts 32 Cationic peroxidase precursor BG584470 (E) 7

rts 33 Isoflavone reductase BG645198 (E) 13

rts 36 Isoflavone reductase homolog BI312226 E/Mt, (E) 5

rts 39 Acidic glucanase^a BF650084 (E), E/Mt, N 4

rts 40 Cytochrome c oxidase subunit 6b-1 BI310278 (E), E/Mt 8

rts 41 Gluco endo-1,3-beta-d-glucosidase BE239884 E/Mt 4

rts 42 Hydroxyacyl glutathione hydrolase^a BG584417 (E) 8

rts 43 Chitinase^a CAA71402 N, S, E/Mt, (E) 9

rts 44 Chitinase^a CAA71402 N, S, E/Mt, (E) 12

rts 45 Chitinase^a CAA71402 N, S, E/Mt, (E) 10

rts 46 Cys proteinase BI269594 (E), E/Mt 4

rts 47 Cys proteinase precursor BG645760 (E), E/Mt 6

rts 48 Ascorbate peroxidase BG648814 (E) 4

rts 51 Ascorbate peroxidase P48534 S, N, E/Mt, (E) 4

rts 52 Triose phosphate isomerase BG584164 (E), E/Mt 11

rts 53 In2-1 protein^a BF635446 E/Mt, (E) 7

rts 54 Uridylate kinase (UDP kinase) AW981222 E/Mt, (E) 4

rts 56 Chalcone-flavone isomerase AW559891 (E), E/Mt, N, S 6

rts 60 Unknown protein^a AW686250 (E), E/Mt 9

rts 61 Alpha fucosidase^a BE942130 (E) 6

rts 63 Putative protein T25B15.70^a BF520168 E/Mt, (E) 8

rts 65 Seed protein precursor AL371551 E/Mt, (E) 4

rts 66 Vc Cyp (peptidyl isomerase) BE316900 (E), E/Mt 4

rts 69 Profucosidase^a AW126318 (E) 5

rts 75 Putative protein^a BF005271 (E), E/Mt 8

rts 77 Glyceraldehyde-3-phosphate dehydrogenase BF635050 E/Mt, (E), N 9

rts 79 Cu/Zn superoxide dismutase^a AL387737 E/Mt 4

rts 80 aba-Responsive protein ABR17 BF648027 E/Mt, (E) 8

rts 81 Unknown protein^a AL365549 E/Mt, (E) 6

rts 82 Putative ripening-related protein^a BE943167 E/Mt, (E) 4

rts 92 Thioredoxin BE997543 (E), E/Mt 4

flw 3 Valosin-containing cell division protein P54774 S, N 16

flw 6 NADH ubiquinone oxidoreductase AW587332 E/Mt, (E) 6

flw 7 Heat shock 70 Q02028 N, S 10

flw 8 Poly(A⁺)-binding protein^a BG584083 (E) 5

flw 11 Phosphoglyceromutase BG585916 (E), E/Mt, N, S 10

flw 13 Putative methyl-binding domain AL378817 (E), E/Mt 5

flw 14 Calreticulin AW773889 E/Mt 4/(348)

flw 17 ATPase beta subunit CAA75477 N, S, E/Mt, (E) 8

flw 18 Enolase CAB75428 N, S, E/Mt, (E) 7

flw 21 S-adenosyl Met synthetase AAL16064 N, S, E/Mt, (E) 8

flw 23 Rubisco activase AAK25798 N, S, E/Mt, (E) 9

flw 27 Ankyrin repeat protein HBP1 BI311773 (E) 9

flw 26 Fru-1,6-biphosphate aldolase O65735 N, S, E/Mt, (E) 7

flw 28 Aspartate aminotransferase P46643 S, N, E/Mt 4/(115)

flw 31 1-Aminocyclopro. carboxylic acid oxidase^a AY062251 N, (E) 6

flw 32 Pyruvate dehydrogenase beta unit^a BF645846 (E), E/Mt 6

flw 30 Glyceraldehyde-3-phosphate dehydrogenase P34922 N, S, E/Mt, (E) 8

flw 33 Malate dehydrogenase O48905 S, N 4

flw 34 Malate dehydrogenase O48905 N, S, (E) 8

flw 35 Ripening-induced protein^a BI308422 (E) 8

flw 39 Cytochrome c oxidase subunit 6b BI310278 (E), E/Mt 5

flw 40 Stromal ascorbate peroxidase Z67113 (E), E/Mt 7

flw 50 Acid phosphatase BG588612 (E), E/Mt 7

flw 51 Acid phosphatase BF004054 (E), E/Mt 6

flw 53 Triose phosphate isomerase BG584164 (E) 11

flw 55 Osmotin-like protein BI270608 (E) 8

flw 56 Chalcone isomerase BI310352 (E), E/Mt 6

flw 57 Ascorbate peroxidase AAL15164 N, S, E/Mt, (E) 5

flw 60 Oxygen-evolving enhancer protein 2 BF636854 E/Mt, (E) 5

flw 71 Peptidyl prolyl isomerase BE999037 (E), E/Mt 5

flw 73 Acid phosphatase AW584917 (E), E/Mt 4/(131)

flw 74 Peptidyl prolyl isomerase BE997455 (E), E/Mt 6

flw 75 Gly-rich RNA binding protein BF637655 E/Mt 4

flw 77 Peroxiredoxin (peroxidase) AW585033 (E), E/Mt 12

flw 78 Ubiquitin-like SMT3 protein AL376595 (E), E/Mt, S 8

flw 79 Ubiquitin-like SMT3 protein P55852 S, N, E/Mt mt 7

flw 81 60S acidic ribosomal protein p3 BF003585 (E), E/Mt 4/(123)

flw 82 Gly cleavage system h precursor^a BF518986 (E), E/Mt 5

flw 88 Acid ribosomal protein P2a2 AW329482 (E) 5

flw 91 Immunophilin AW574158 (E), E/Mt 6

flw 92 Rubisco small chain BI268542 (E), E/Mt 5

flw 94 Profilin 1^a AL373653 (E) 9

flw 96 NADH plastoquinone oxidoreductase 4^a BF631701 (E) 6

pds 5 Convicilin^a BI312063 (E) 5

pds 6 Convicilin^a BI310979 E/Mt 10

pds 7 Heat shock 70 Q02028 N, S 9

pds 8 Legumin a2 precursor^a BI312252 E/Mt 9

pds 9 Protein disulfide isomerase P29828 N, S, (E), E/Mt 7

pds 10 Glycinin^a BI308459 (E) 7

pds 13 Legumin a2 precursor^a BI311943 (E) 9

pds 12^b Rubisco AAK70985 N, S 8

pds 12^b NAp1p (plasma membrane intrinsic protein)^a AW774263 E/Mt, (E) 10

pds 14 Vicilin 47kD precursor^a BI310576 (E) 9

pds 15 Provicilin precursor^a BI312400 (E) 6

pds 16 Vicilin 47kD precursor^a BI311712 (E) 4

pds 19 Vicilin 47kD precursor^a BI311712 (E) 7

pds 22 Glycinin^a BI311592 (E) 13

pds 20 Glycinin^a BI311729 (E) 4

pds 21 Glycinin^a BI308883 (E) 12

pds 23 Glycinin^a BI308883 (E) 6

pds 24 Legumin a2 precursor^a BI309500 (E) 11

pds 28 Legumin a2 precursor^a BI311943 (E) 14

pds 27 Legumin a2 precursor^a BI311943 (E) 11

pds 31 Fru 1,6-biphosphate aldolase O65735 N, S, (E), E/Mt 7

pds 30 Legumin a2 precursor^a BI311943 (E) 11

pds 29 Legumin a2 precursor^a BI311943 (E) 8

pds 32 Legumin a2^a BI307938 (E) 5

pds 34 Cytosolic malate dehydrogenase BG583001 (E), N, S 6

pds 38 Malate dehydrogenase precursor AW688679 E/Mt, N 7

pds 37 Glycinin^a BI311164 (E) 10

pds 39 IFR-like NADH-dependent oxidoreductase BE325778 E/Mt 7

pds 41 Peroxidase 2 CAC38106 N, E/Mt 16

pds 43^b Rubisco CAA62888 N, S 10

pds 43^b Enolase BG362941 E/Gm 7

pds 44 Vicilin 47-kD precursor^a BI310576 (E) 9

pds 47 Acid phosphatase BF004054 E/Mt 5

pds 46 Acid phosphatase BF004054 E/Mt 7

pds 51 Acid phosphatase BG588612 (E) 9

pds 52 Proteosome 20S subunit BE922062 E/St 7

pds 53 Ascorbate peroxidase BG648703 (E), E/Mt 10

pds 54 Osmotin like protein precursor BG582096 (E), E/Mt 8

pds 55 Putative GSH-dependent dehydroascorbate reductase^a BF636747 E/Mt, (E) 6

pds 56 Legumin a2 precursor^a BI307938 (E) 7

pds 57 Legumin a2 precursor^a BI309895 (E) 6

pds 58 Oxygen-evolving enhancer protein 2 AW775879 E/Mt, (E), S 12

pds 59 Legumin a2 precursor^a BI309155 (E) 9

pds 62 Legumin b (minor small)^a BI311720 (E) 9

pds 65 Legumin b (minor small)^a BI311720 (E) 12

pds 66 Legumin b (minor small)^a BI311437 (E) 6

pds 68 Legumin-related high-M_r polypeptide^a BI310430 (E) 8

pds 70 Hypothetical protein^a AW685677 E/Mt, (E) 9

pds 71 Vicilin 4-kD precursor^a BI312335 (E) 10

pds 75 LEA protein^a BG454568 (E) 4

pds 73 Legumin a2 precursor^a BI309500 (E) 4

pds 72 Eukaryotic initiation factor 5a^a AL389124 E/Mt 4

pds 77 VcCyP peptidylprolyl isomerase BE997455 E/Mt, (E) 10

pds 78 VcCyP peptidylprolyl isomerase BE997455 E/Mt, (E) 13

pds 80 Ubiquitin-like protein AL376595 E/Mt 5

pds 82 aba-Responsive protein abr 17 BF648027 (E) 5

pds 84 Gly-rich RNA-binding protein BI309824 (E), E/Mt 11

pds 85 Acidic ribosomal protein AL383563 E/Mt 5

pds 91 Legumin (minor small)^a BI311437 (E) 7

pds 95 Rubisco small subunit BF519894 E/Mt 8

pds 94 Plastocyanin precursor BF005687 E/Mt 6

cls 2 Cell division cycle prt 48 (valosin contain. prt) P54774 S 10

cls 5 Heat shock protein 70-kD (Bip A) T06598 E/Hv, N, S 10

cls 6 Luminal-binding protein CAC14168 N, S, E/Hv 8

cls 7 Psst 70 Q02028 N, S, E/Hv 9

cls 8 Putative-luminal binding protein CAC14168 N, S, (E) 9

cls 10 Leucyl aminopeptidase^a S57811 N, S 4

cls 9 70-kD heat shock protein Q01899 N, S, E/Mt, (E) 8

cls 20 Catalase^a P49315 N, S, (E) 6

cls 15 Selenium-binding protein^a CAC67501 N, (E) 12

cls 16 Selenium-binding protein^a CAC67501 N, (E) 7

cls 14^b Calreticulin Q40401 N, E/Mt 4/(135)

cls 14^b Nucleosome assembly protein 1^a S60893 E/Mt NA/(208)

cls 18 ATP synthase beta subunit CAA75478 N, S, E/Mt, (E) 6

cls 19 Inosine-5'-monophosphate dehydrogenase^a AAL18815 N, E/Mt, (E) 5

cls 21 Hydroxymethyltransferase^a AW980652 E/Mt, (E) 4

cls 22 Enolase CAB75428 N, E/Mt 6

cls 23 SAM synthetase AAG17666 N, E/Mt, (E) 5

cls 24^b Glc-6-phosphate 1 dehydrogenase Q42919 S 5

cls 24^b SAM synthetase 2 Q96552 N, E/Mt, E/Gm 5

cls 28 Aspartate aminotransferase P28011 N, S, E/Mt 17

cls 29 Putative heat shock protein AAK63929 N 5

cls 30 12-Oxophytodienoic acid 10,11-reductase^a BG648922 E/Mt, (E) 6

cls 31 12-Oxophytodienoate reductase (OPR2)^a AW776305 E/Mt 6

cls 27 RAD23 (ubiquitin-like protein)^a AW586882 (E) 4

cls 33 Probable mannitol dehydrogenase AW981164 (E) 6

cls 32 Alcohol dehydrogenase^a P12886 S, (E) 5

cls 35^b Catalase^a P45739 S 5

cls 35^b Fru-1,6-biphosphate aldolase P46257 N, S, E/Mt 5

cls 37 2-Nitropropane dioxygenase-like protein^a BF518520 E/Mt, (E) 7

cls 39 Fructokinase AW584645 E/Mt, N, S 8

cls 46 Beta-1,3-glucanase BF650622 E/Mt 4/(378)

cls 49 Stromal L-ascorbate peroxidase precursor BE941206 E/Mt, (E) 5

cls 53 Cytochrome b5 reductase^a NP 568391 E/Mt NA/(406)

cls 60 Glyceraldehyde-3-phosphate dehydrogenase P54270 N, S 5

cls 62 Rubisco, small subunit PS6577 S 4

cls 64 Proteosome subunit $alpha$ -type 5 (20S subunit) Q9M4T8 E/Mt, N, S NA/(449)

cls 67 NADH ubiquinone oxidoreductase BG448277 (E) 4

cls 74 vcCyp (peptidylprolyl isomerase) AW775250 E/Mt 9

cls 78 Peroxiredoxin TPx1 (thioredoxin peroxidase) AW559683 (E), E/Mt 11

cls 81 Peptidylprolyl isomerase (immunophilin) BF635887 E/Mt, (E) 5

cls 83 Disease resistance response protein^a BE942549 E/Mt, S 9

cls 82^b aba-Responsive protein BF648027 E/Mt, (E) 10

cls 82^b Leghemoglobin 2 (Pprg2)^a P27993 S 4/(680)

cls 87 Class 10 PR protein^a Q43560 N, S, E/Mt 5

cls 85 Cytochrome C-555^a P00124 S 5

cls 88 Nucleoside diphosphate kinase P47922 S 7

cls 86 Gly-rich RNA binding protein AAF06329 E/Mt, N 9

cls 90 Immunophilin AL377066 E/Mt 4

cls 92 Acidic ribosomal protein (60S) AL378424 E/Mt 5

cls 96 10-kD chaperonin^a AL377948 E/Mt 4/(121)

^a Putative unique protein identified only in one tissue. ^b Multiple proteins identified in this 2-DE spot.