This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Xia, J.-H. Articles by Saglio, P. H. Search for Related Content PubMed PubMed Citation Articles by Xia, J.-H. Articles by Saglio, P. H. Agricola Articles by Xia, J.-H. Articles by Saglio, P. H.

Environmental and Stress Physiology

Lactic Acid Efflux as a Mechanism of Hypoxic Acclimation of Maize Root Tips to Anoxia

Station de Physiologie Végétale, Institut National de la Recherche Agronomique, B.P. 81, 33883 Villenave d'Ornon Cedex, France, Centre de Recherches de Bordeaux, B.P. 81, 33883 Villenave d'Ornon Cedex, France

Hypoxic pretreatment (3 kPa oxygen) of maize (Zea mays L.) root tips improved their survival time in a subsequent anoxic incubation from 10 h to more than 3 d, provided that glucose was added to the medium to sustain metabolism. The glycolytic flux (lactate + ethanol) was the same in both pretreated and untreated root tips during the 1st h after transfer to anoxia. It was only after 2 h that it declined sharply in untreated tips, but was sustained in pretreated ones. Right after the transition from normoxia to anoxia of untreated root tips, the only fermentative product detected was lactic acid, which accumulated in a 7:1 proportion after 30 min in tissue and medium, respectively. It took 10 min before ethanol could be detected and 20 min for it to be produced at its maximum rate at the expense of lactate production, which slowed down. In contrast, in hypoxically pretreated root tips, ethanol was produced at a maximum rate right after the transfer to anoxia. Concurrently, low amounts of lactic acid were produced that accumulated in a 1:1 proportion after 30 min in tissue and medium, respectively. This large efflux of lactic acid could account for the higher cytoplasmic pH values always found in pretreated tissues. The presence of cycloheximide during pretreatment abolished this difference, suggesting that the greater efficiency of lactate efflux was linked to protein synthesis. The role of lactate in cytosolic pH regulation and in sensitivity to anoxia is discussed.

This article has been cited by other articles:

				L. Magneschi and P. Perata Rice Germination and Seedling Growth in the Absence of Oxygen Ann. Bot., July 25, 2008; (2008) mcn121v1. [Abstract] [Full Text] [PDF]

				H. H. FELLE pH Regulation in Anoxic Plants Ann. Bot., September 1, 2005; 96(4): 519 - 532. [Abstract] [Full Text] [PDF]

				S. Huang, K. Ishizawa, H. Greenway, and T. D. Colmer Manipulation of ethanol production in anoxic rice coleoptiles by exogenous glucose determines rates of ion fluxes and provides estimates of energy requirements for cell maintenance during anoxia J. Exp. Bot., September 1, 2005; 56(419): 2453 - 2463. [Abstract] [Full Text] [PDF]

				S. ASCHI-SMITI, W. ChAIBI, R. BROUQUISSE, B. RICARD, and P. SAGLIO Assessment of Enzyme Induction and Aerenchyma Formation as Mechanisms for Flooding Tolerance in Trifolium subterraneum 'Park' Ann. Bot., January 2, 2003; 91(2): 195 - 204. [Abstract] [Full Text] [PDF]

				H.-P. Peng, C.-S. Chan, M.-C. Shih, and S. F. Yang Signaling Events in the Hypoxic Induction of Alcohol Dehydrogenase Gene in Arabidopsis Plant Physiology, June 1, 2001; 126(2): 742 - 749. [Abstract] [Full Text] [PDF]

				E. Gout, A.-M. Boisson, S. Aubert, R. Douce, and R. Bligny Origin of the Cytoplasmic pH Changes during Anaerobic Stress in Higher Plant Cells. Carbon-13 and Phosphorous-31 Nuclear Magnetic Resonance Studies Plant Physiology, February 1, 2001; 125(2): 912 - 925. [Abstract] [Full Text]

				A. Chabbi, K. L. McKee, and I. A. Mendelssohn Fate of oxygen losses from Typha domingensis (Typhaceae) and Cladium jamaicense (Cyperaceae) and consequences for root metabolism Am. J. Botany, August 1, 2000; 87(8): 1081 - 1090. [Abstract] [Full Text]

				W. W.P. Chang, L. Huang, M. Shen, C. Webster, A. L. Burlingame, and J. K.M. Roberts Patterns of Protein Synthesis and Tolerance of Anoxia in Root Tips of Maize Seedlings Acclimated to a Low-Oxygen Environment, and Identification of Proteins by Mass Spectrometry Plant Physiology, February 1, 2000; 122(2): 295 - 318. [Abstract] [Full Text]

				Y. Zeng, Y. Wu, W. T. Avigne, and K. E. Koch Rapid Repression of Maize Invertases by Low Oxygen. Invertase/Sucrose Synthase Balance, Sugar Signaling Potential, and Seedling Survival Plant Physiology, October 1, 1999; 121(2): 599 - 608. [Abstract] [Full Text]

				M. H. Ellis, E. S. Dennis, and W. James Peacock Arabidopsis Roots and Shoots Have Different Mechanisms for Hypoxic Stress Tolerance Plant Physiology, January 1, 1999; 119(1): 57 - 64. [Abstract] [Full Text]

				A. W. Sowa, S. M. G. Duff, P. A. Guy, and R. D. Hill Altering hemoglobin levels changes energy status in maize cells under hypoxia PNAS, August 18, 1998; 95(17): 10317 - 10321. [Abstract] [Full Text] [PDF]

				B. Ricard, T. V. Toai, P. Chourey, and P. Saglio Evidence for the Critical Role of Sucrose Synthase for Anoxic Tolerance of Maize Roots using a Double Mutant Plant Physiology, April 1, 1998; 116(4): 1323 - 1331. [Abstract] [Full Text]

				M. Dieuaide-Noubhani, Gér. Raffard, P. Canioni, A. Pradet, and P. Raymond Quantification of Compartmented Metabolic Fluxes in Maize Root Tips Using Isotope Distribution from [IMAGE]C- or [IMAGE]C-Labeled Glucose J. Biol. Chem., June 2, 1995; 270(22): 13147 - 13159. [Abstract] [Full Text] [PDF]