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Plant Physiology 100:525-528 (1992)
© 1992 American Society of Plant Biologists

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Metabolism and Enzymology

Induction of Glutamine Synthetase Activity in Nonnodulated Roots of Glycine max, Phaseolus vulgaris, and Pisum sativum1

Inger Hoelzle, John J. Finer, Michael D. McMullen and John G. Streeter

Agronomy Department, The Ohio State University/Ohio Agricultural Research and Development Center, Corn and Soybean Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Wooster, Ohio 44691

Nitrate or ammonium fertilization significantly increased glutamine synthetase (GS) activity in nonnodulated roots of French bean (Phaseolus vulgaris), soybean (Glycine max), and pea (Pisum sativum). Western analysis revealed substantial GS antibody-positive protein in root extracts that had minimal GS activity, indicating that an inactive form of GS may be present in nonfertilized plants.

1 Supported by The Ohio State University (I.H., J.J.F., J.G.S.) and the U.S. Department of Agriculture, Agricultural Research Service (M.D.M.). Salary for I.H. was from project No. 245627. This is Ohio Agricultural Research and Development Center paper No. 78-92.




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Copyright © 1992 by the American Society of Plant Biologists