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Plant Physiology 100:529-532 (1992)
© 1992 American Society of Plant Biologists

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Metabolism and Enzymology

A New Method of Measuring Protein-Methionine-S-Oxide Reductase Activity 1

David L. Ferguson and John J. Burke

U.S. Department of Agriculture, Route 3, Box 215, Lubbock, Texas 79401, Plant Stress and Water Conservation Research Unit, Route 3, Box 215, Lubbock, Texas 79401

A new assay for measuring protein-methionine-S-oxide reductase is reported. The assay measures the conversion of N-(9-fluorenylmethoxycarbonyl)-methionine sulfoxide to N-(9-fluorenylmethoxycarbonyl)-methionine using fluorescence detection after high-performance liquid chromatography separation. Enzyme activity is linear over a 60-min period and the assay is sensitive enough to detect the consumption of only 1% of available substrate. Protein-methionine-S-oxide reductase activity was demonstrated in pea (Pisum sativum L.) chloroplasts, and enzyme levels in leaves of etiolated and light-grown seedlings were compared.

1 This work was supported by grant 90-37280-5462 from the National Research Institute Competitive Grants Program/U.S. Department of Agriculture.




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Copyright © 1992 by the American Society of Plant Biologists