Plant Physiology 100:593-596 (1992)
© 1992 American Society of Plant Biologists
Environmental and Stress Physiology
Antifreeze Protein Produced Endogenously in Winter Rye Leaves 1
Marilyn Griffith,
Paul Ala,
Daniel S. C. Yang,
Wai-Ching Hon and
Barbara A. Moffatt
Department of Biology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada,
Department of Biochemistry, McMaster University, Hamilton, Ontario L8N 3Z5, Canada
After cold acclimation, winter rye (Secale cereale L.) is able to withstand the formation of extracellular ice at freezing temperatures. We now show, for the first time, that cold-acclimated winter rye plants contain endogenously produced antifreeze protein. The protein was extracted from the apoplast of winter rye leaves, where ice forms during freezing. After partial purification, the protein was identified as antifreeze protein because it modified the normal growth pattern of ice crystals and depressed the freezing temperature of water noncolligatively.
1 This work was supported by the Natural Science and Engineering Research Council of Canada through operating grants to M.G. and B.A.M. and by a Medical Research Council of Canada grant to D.S.C.Y.
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