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Plant Physiology 100:778-783 (1992)
© 1992 American Society of Plant Biologists

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Metabolism and Enzymology

Basic Endochitinases Are Major Proteins in Castanea sativa Cotyledons 1

Carmen Collada, Rosa Casado, Aurora Fraile and Cipriano Aragoncillo

Departamento de Bioquímica, Escuela Técnica Superior Ingenieros de Montes, 28040 Madrid, Spain, Departamento de Patología Vegetal, Escuela Técnica Superior Ingenieros Agrónomos, 28040 Madrid, Spain

Basic endochitinases are abundant proteins in Castanea sativa Mill. cotyledons. Three basic chitinases were purified with molecular masses of 25, 26, and 32 kD (Ch1, Ch2, and Ch3) and with isoelectric points between 8 and 9.5. Antibodies raised against Ch1 cross-reacted with Ch2 and Ch3. However, Ch3 showed differences when compared with the other two enzymes, especially in its higher cysteine content. The size, amino acid composition, and N-terminal sequence of Ch1 indicate that it is a class II endochitinase and, therefore, has no cysteine-rich hevein domain. Ch1 inhibits the growth of the fungus Trichoderma viride. The biological role of these endochitinases is discussed.

1 This work was supported by grant FOR 90-0151 from the Comisión Interministerial de Ciencia y Tecnología.




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M.-A. Lopez-Matas, P. Nunez, A. Soto, I. Allona, R. Casado, C. Collada, M.-A. Guevara, C. Aragoncillo, and L. Gomez
Protein Cryoprotective Activity of a Cytosolic Small Heat Shock Protein That Accumulates Constitutively in Chestnut Stems and Is Up-Regulated by Low and High Temperatures
Plant Physiology, April 1, 2004; 134(4): 1708 - 1717.
[Abstract] [Full Text] [PDF]


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Copyright © 1992 by the American Society of Plant Biologists