Plant Physiology 100:1087-1091 (1992)
© 1992 American Society of Plant Biologists
Membranes and Bioenergetics
Measurements of the Engagement of Cyanide-Resistant Respiration in the Crassulacean Acid Metabolism Plant Kalanchoë daigremontiana with the Use of On-Line Oxygen Isotope Discrimination 1
Sharon A. Robinson2,
Dan Yakir3,
Miquel Ribas-Carbo4,
Larry Giles,
C. Barry Osmond2,
James N. Siedow and
Joseph A. Berry
Department of Botany, Duke University, Durham, North Carolina 27706,
Carnegie Institution of Washington, Department of Plant Biology, 290 Panama Street, Stanford, California 94305-1297
Discrimination against 18O during dark respiration in tissues of Kalanchoë daigremontiana, Medicago sativa, and Glycine max was measured using an on-line system that enabled direct measurements of the oxygen fractionation of samples in a gas-phase leaf disk electrode unit. Discrimination factors for cytochrome pathway respiration were 18.6 to 19.8%o for all tissues. However, discrimination in cyanide-resistant respiration was significantly higher in green tissues (30.4-31.2%o) compared with nongreen tissues (25.3-25.9%o). Using these discrimination factors, the partitioning of electron transport to these pathways was calculated from measurements of discrimination in the absence of inhibitors. Changes in flux through the alternative pathway were measured during the light and dark phases of Crassulacean acid metabolism in leaf disks of K. daigremontiana. The flux of electrons through the alternative pathway was higher during deacidification than during the other phases of Crassulacean acid metabolism. The increase in alternative pathway electron flux accounted for all of the increased respiration in the light phase. Despite this increase, simultaneous measurements of malate concentration and respiratory flux confirm that only a small proportion of the total malate decarboxylation occurs in the mitochondria.
2 Permanent address: Research School of Biological Sciences, The Australian National University, Box 475, Canberra ACT 2601 Australia.
3 Permanent address: Weizmann Institute of Science, Department of Environmental Science and Energy Research, Rehovot, Israel 76100.
4 Permanent address: Department de Fisiologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain 08028.
1 This work was supported by National Science Foundation (NSF) grant DCB90-06830 and Department of Energy, Division of Energy Biosciences grant DE-FG05-89 ER 14005 (C.B.O.), Binational Agricultural Research and Development Fund project IS-1344-87 (J.A.B.), Comision Interministerial para la Ciencia y Tecnologia (AGR89-580) (M.R.C.), and NSF grant BSR 87-06429 to the Duke University Phytotron. Carnegie Institution of Washington, Department of Plant Biology Publication No. 1149; Weizmann Institute Department of Environmental Science and Energy Research Publication No. 68.
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