Plant Physiol. Drug Metab Dispos
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Plant Physiology 100:1114-1119 (1992)
© 1992 American Society of Plant Biologists

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Metabolism and Enzymology

Induction of UDP-Glucose:Salicylic Acid Glucosyltransferase in Oat Roots 1

Nasser Yalpani2, Nelson E. Balke and Margot Schulz3

Department of Agronomy, University of Wisconsin, Madison, Wisconsin 53706

A UDP-glucose:salicylic acid 3-O-glucosyltransferase (EC 2.4.1.35) (GTase) from oat (Avena sativa L. cv Dal) root extracts was assayed in vitro using [14C]salicylic acid (SA) and an ion exchange column to separate SA from beta-glucosylsalicylic acid. The GTase, present at a very low constitutive level, was inducible to 23 times the constitutive level. When excised roots were exposed to SA at pH 6.5, the specific activity of the enzyme increased within 1.5 h, peaked after 8 to 10 h, and then declined. The increase in specific activity depended on the concentration of SA in the induction medium. Among 16 phenolics and phenolic derivatives tested, GTase induction showed high specificity toward SA and acetylsalicylic acid. Specific activity of the enzyme was induced to higher levels in roots from 7-d-old seedlings than roots from younger plants. GTase activity was less inducible in basal compared with median or apical root sections. Induction of GTase activity was a result of de novo RNA and protein synthesis. Candidate peptides for the GTase were identified by comparison of two-dimensional electrophoresis gels of proteins labeled with [35S]methionine during incubation of roots in the presence or the absence of SA and a gel of a partially purified GTase preparation.

2 Present address: Center for Agricultural Molecular Biology, Rutgers, The State University of New Jersey, Cook College, P.O. Box 231, New Brunswick, NJ 08903-0231.

3 Present address: Institut fur Landwirtschaftliche Botanik, Meckenheimer Allee 176, D-5300 Bonn, Federal Republic of Germany.

1 Support to N.E.B. by U.S. Department of Agriculture Competitive Research Grants Office grant No. 85-CRCR-1-1572, University of Wisconsin-Madison Graduate School project No. 900317, and the College of Agricultural and Life Sciences, University of Wisconsin-Madison.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1992 by the American Society of Plant Biologists