Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 100:1377-1383 (1992)
© 1992 American Society of Plant Biologists

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Development and Growth Regulation

Effect of Brassinolide on Gene Expression in Elongating Soybean Epicotyls 1

Steven D. Clouse, Daniel M. Zurek, Trevor C. McMorris and Michael E. Baker

Department of Biology, San Diego State University, San Diego, California 92182, Department of Chemistry, University of California, San Diego La Jolla, California 92093, Department of Medicine, University of California, San Diego La Jolla, California 92093

We have studied the effect of brassinolide (BR), a plant steroidal lactone, on the expression of auxin-regulated genes in soybean (Glycine max L. cv Williams 82) epicotyls. BR caused up to 4-fold increases in epicotyl length during extended assays at 10–7M, in the absence of added auxin. Structurally related steroids failed to induce elongation or to alter the BR effect. Northern blot analysis, using sequences corresponding to auxin-regulated genes as probes, has shown that the molecular mechanism of BR-induced elongation is likely to differ from that of auxin-induced elongation in this system. BR does not rapidly induce members of the GH, SAUR, or JCW auxin-inducible gene families before the onset of elongation. BR enhances SAUR and GH1 transcripts after 18 h but has no effect on JCW1 or GH3 transcripts at any time examined. We have shown by two-dimensional gel analysis of in vitro translated mRNA that a submicromolar concentration of BR alters the pattern of gene expression in elongating soybean epicotyls.

1 This research was supported in part by U.S. Department of Agriculture/Competitive Research Grant Office grant No. 90-37261-5700 and National Science Foundation grant No. DCB-9013409 to S.D.C.




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