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Environmental and Stress Physiology

Induction of a Putative Ca²⁺-ATPase mRNA in NaCl-Adapted Cells ¹

Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana 47907-1165, Facultad de Ciencias, Departamento de Bioquimica y Biologia Molecular, Universidad de Malaga, 29071 Malaga, Spain, Texas A&M University Agricultural Research and Extension Center, 1380 A&M Circle, El Paso, Texas 79927

A cDNA clone was isolated that encodes the partial sequence of a putative endoplasmic reticulum Ca²⁺-ATPase of tobacco. The 1.497-kb insert had an open reading frame of 1.149 kb. The deduced peptide had the greatest homology to the endoplasmic reticulum Ca²⁺-ATPases of Drosophila and Artemia, followed by the mammalian and avian enzymes (SERCA2 and 3). The cDNA insert hybridized to a single mRNA of 4.4 kb from tobacco cultured cells or plant tissues. The level of this transcript was induced about 2-fold by NaCl shock in 428 mM NaCl-deadapted tobacco cells that were maintained in medium without salt, but not in unadapted cells. The level of this transcript was 3- to 4-fold higher in 428 mM NaCl-adapted cells growing in salt than in unadapted cells growing without salt.

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