Plant Physiology 100:1503-1507 (1992)
© 1992 American Society of Plant Biologists
Molecular Biology and Gene Regulation
Expression of a Maize Ubiquitin Gene Promoter-bar Chimeric Gene in Transgenic Rice Plants 1
Seiichi Toki,
Susumu Takamatsu,
Chyuhei Nojiri,
Shinya Ooba,
Hiroyuki Anzai,
Michiaki Iwata,
Alan H. Christensen2,
Peter H. Quail and
Hirofumi Uchimiya
Department of Botany, Faculty of Science, Hokkaido University, Sapporo 060, Japan,
Fukui Agricultural Experiment Station, Fukui City 910, Japan,
Meiji Seika Ltd. Pharmaceutical Research Center, Yokohama 222, Japan,
Institute of Applied Microbiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan,
University of California, Berkeley/United States Department of Agriculture Plant Gene Expression Center, Albany, California 94710
We have constructed a chimeric gene consisting of the promoter, first exon, and first intron of a maize ubiquitin gene (Ubi-1) and the coding sequence of the bar gene from Streptomyces hygroscopicus. This construct was transferred into rice (Oryza sativa L.) protoplasts via electroporation, and 10 plants were regenerated from calli that had been selected for resistance to exogenously supplied bialaphos. Transgenic plants grown in a greenhouse were resistant to both bialaphos and phosphinothricine at a dosage lethal to untransformed control plants. Evidence of stable integration of the transferred gene into the genome of the regenerated primary transformant plants was obtained from Southern blot analysis. In addition, northern blot analysis indicated expression and proper splicing of the maize ubiquitin gene first intron from the primary chimeric transcript in these transgenic rice plants, and western blot analysis and enzymic assays verified expression of the active bar gene product. Apparent mendelian segregation for bialaphos resistance in T1 progeny of primary transformants was confirmed.
2 Present address: Department of Biology, George Mason University, Fairfax, VA 22030-4444.
1 This research was supported by grants from the Ministry of Education, Science, and Culture of Japan (H.U.), the Ministry of Agriculture, Forestry and Fishery of Japan (H.U.), Suhara Memorial Foundation (H.U., Se.T.), Torey Science Foundation (H.U.), and the Rockefeller Foundation (H.U.). Work in the laboratory of P.H.Q. was supported by a grant from Agrigenetics Research Associates and U.S. Department of Agriculture/Agricultural Research Service No. 5335-23000-004-00D.
This article has been cited by other articles:
|
|
|
|
 
T. Yamada, F. Matsuda, K. Kasai, S. Fukuoka, K. Kitamura, Y. Tozawa, H. Miyagawa, and K. Wakasa
Mutation of a Rice Gene Encoding a Phenylalanine Biosynthetic Enzyme Results in Accumulation of Phenylalanine and Tryptophan
PLANT CELL,
May 1, 2008;
20(5):
1316 - 1329.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. W. Bae, E. Vanjildorj, S. Y. Song, S. Nishiguchi, S. S. Yang, I. J. Song, T. Chandrasekhar, T. W. Kang, J. I. Kim, Y. J. Koh, et al.
Environmental Risk Assessment of Genetically Engineered Herbicide-Tolerant Zoysia japonica
J. Environ. Qual.,
January 4, 2008;
37(1):
207 - 218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shimono, S. Sugano, A. Nakayama, C.-J. Jiang, K. Ono, S. Toki, and H. Takatsuji
Rice WRKY45 Plays a Crucial Role in Benzothiadiazole-Inducible Blast Resistance
PLANT CELL,
June 1, 2007;
19(6):
2064 - 2076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ariizumi, S. Kishitani, R. Inatsugi, I. Nishida, N. Murata, and K. Toriyama
An Increase in Unsaturation of Fatty Acids in Phosphatidylglycerol from Leaves Improves the Rates of Photosynthesis and Growth at Low Temperatures in Transgenic Rice Seedlings
Plant Cell Physiol.,
July 15, 2002;
43(7):
751 - 758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. FUKUZAWA, S. ARAI, M. KAWAI-YAMADA, A. DAS, M. TAGAWA, and H. UCHIMIYA
Glufosinate-tolerant Tobacco Plants Directed by the Promoter of Adenylate Kinase Gene of Rice
Ann. Bot.,
March 1, 2002;
89(3):
351 - 354.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-R. Scheible, R. Eshed, T. Richmond, D. Delmer, and C. Somerville
Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in Arabidopsis Ixr1 mutants
PNAS,
August 17, 2001;
(2001)
191361598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Moutinho, P. J. Hussey, A. J. Trewavas, and R. Malho
cAMP acts as a second messenger in pollen tube growth and reorientation
PNAS,
August 17, 2001;
(2001)
171104598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Werner, V. Motyka, M. Strnad, and T. Schmulling
Regulation of plant growth by cytokinin
PNAS,
August 10, 2001;
(2001)
171304098.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Tozawa, H. Hasegawa, T. Terakawa, and K. Wakasa
Characterization of Rice Anthranilate Synthase {alpha}-Subunit Genes OASA1 and OASA2. Tryptophan Accumulation in Transgenic Rice Expressing a Feedback-Insensitive Mutant of OASA1
Plant Physiology,
August 1, 2001;
126(4):
1493 - 1506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Jiang, S. D. Linscombe, J. Wang, and J. H. Oard
High Efficiency Transformation of U.S. Rice Lines from Mature Seed-Derived Calli and Segregation of Glufosinate Resistance under Field Conditions
Crop Sci.,
November 1, 2000;
40(6):
1729 - 1741.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Cheng, R. Sardana, H. Kaplan, and I. Altosaar
Agrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer
PNAS,
March 17, 1998;
95(6):
2767 - 2772.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Yoshimura, U. Yamanouchi, Y. Katayose, S. Toki, Z.-X. Wang, I. Kono, N. Kurata, M. Yano, N. Iwata, and T. Sasaki
Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation
PNAS,
February 17, 1998;
95(4):
1663 - 1668.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Nayak, D. Basu, S. Das, A. Basu, D. Ghosh, N. A. Ramakrishnan, M. Ghosh, and S. K. Sen
Transgenic elite indica rice plants expressing CryIAc partial -endotoxin of Bacillus thuringiensis are resistant against yellow stem borer (Scirpophaga incertulas)
PNAS,
March 18, 1997;
94(6):
2111 - 2116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
