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Membranes and Bioenergetics

Vanadate Inhibits Blue Light-Stimulated Swelling of Vicia Guard Cell Protoplasts ¹

Department of Biology, University of California, Los Angeles, CA 90024, Laboratory of Biomedical and Environmental Sciences, University of California, Los Angeles, CA 90024

When supplied under low chloride concentrations, vanadate inhibits the blue light-stimulated swelling of Vicia faba L. guard cell protoplasts in a dose-dependent fashion. The volume of guard cell protoplasts incubated in 10 mM K-imino-diacetic acid, 0.4 M mannitol, and 1 mM CaCl₂ remained essentially constant under 1000 µmol m^–2 s^–1 red light, but increased an average of 27% after 8 min of the addition of 50 µmol m^–2 s^–1 blue light to the background red light. At 500 µM, vanadate completely inhibits the response to blue light. Vanadate also inhibits the swelling of guard cell protoplasts stimulated by the H⁺-ATPase agonist fusicoccin. The vanadate sensitivity of the blue light-stimulated swelling implicates a proton-pumping ATPase as a component of the sensory transduction of blue light in guard cells.

¹ This work was supported by a National Science Foundation grant No. DCB 8904254 to E.Z.

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