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Metabolism and Enzymology

Intracellular Localization of Three L-Glutamate Dehydrogenase Isozymes from Chlamydomonas reinhardtii¹

Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, Avda, San Alberto Magno s/n, E-14071-Córdoba, Spain, Institute of Plant Physiology, the Russian Academy of Sciences, Moscow-276, Botanicheskaya 35, Russia, CIS

The intracellular localization of the activity and synthesis of three isozymes of NAD(P)⁺-glutamate dehydrogenase from the unicellular green alga Chlamydomonas reinhardtii cw-92 has been established. Isozyme activities have been located within mitochondria by using differential centrifugation techniques and discontinuous Percoll gradient separations. Experiments with protein synthesis inhibitors cycloheximide, rifampicin, chloramphenicol, and actinomycin D, under dark and carbon starvation conditions, revealed that synthesis of the three isozymes was likely to occur in cytosol as precursor proteins that are then transported and processed inside the mitochondria.

¹ This work was supported by grants from the Comisión Interministerial de Ciencia y Tecnologiá (PB90-0780-CO2-01) and Consejería de Educación y Ciencias (grupo No. 3249), Junta de Andalucía, Spain, to J.C. Z.R. is a fellow of Ministerio de Educación y Ciencia, Spain.