Plant Physiol. Drug Metab Dispos
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Plant Physiology 100:1584-1586 (1992)
© 1992 American Society of Plant Biologists

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Metabolism and Enzymology

In Situ Nuclear Magnetic Resonance of 15N Pulse Labels Monitors Different Routes for Nitrogen Assimilation 1

Rainer Callies2, Rolf Altenburger, Sibylle Abarzua, Adalbert Mayer, L. Horst Grimme and Dieter Leibfritz

Institute für Experimentelle Physik, Universität Bremen, Postfach 330 440, W-2800 Bremen 33, Federal Republic of Germany, Zellbiologie, Biochemie und Biotechnologie, Universität Bremen, Postfach 330 440, W-2800 Bremen 33, Federal Republic of Germany, Organische Chemie, Universität Bremen, Postfach 330 440, W-2800 Bremen 33, Federal Republic of Germany, Sektion Biologie, Wissenschaftsbereich Pflanzenphysiologie und Biochemie, Universität Rostock, Doberaner Strasse 143, O-2500 Rostock, Federal Republic of Germany

Nuclear magnetic resonance offers the possibility of noninvasive in situ observation of 15N pulse labeling in the presence of light. In vivo, exclusively the {delta}-nitrogen of Gln is labeled in the cyanobacterium Microcystis firma when glutamate synthase is inhibited by azaserine. In contrast, the green alga Chlorella fusca is additionally capable of incorporating nitrogen into Glu, thus providing evidence for an anabolic function of glutamate dehydrogenase in this organism.

2 Present address: Department of Biochemistry and Molecular Biology, University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, UK.

1 This work was supported by the Deutsche Forschungsgemeinschaft. R.A. acknowledges the receipt of a scholarship of the Ev. Studienwerk Villigst and S.A. acknowledges a grant of the Deutsche Akademische Austauschdienst. We are grateful to S. P. Williams for revising the English.




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P. S. van Heerden, G. H. N. Towers, and N. G. Lewis
Nitrogen Metabolism in Lignifying Pinus taeda Cell Cultures
J. Biol. Chem., May 24, 1996; 271(21): 12350 - 12355.
[Abstract] [Full Text] [PDF]


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Copyright © 1992 by the American Society of Plant Biologists