This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Carvalho, H. Articles by Salema, R. Search for Related Content PubMed PubMed Citation Articles by Carvalho, H. Articles by Salema, R. Agricola Articles by Carvalho, H. Articles by Salema, R.

Metabolism and Enzymology

Detection of a Cytosolic Glutamine Synthetase in Leaves of Nicotiana tabacum L. by Immunocytochemical Methods ¹

Centro de Citologia Experimental (Instituto Nacional de Investigação Científica), Universidade do Porto, 4100-Porto, Portugal, Instituto de Botânica, Universidade do Porto, 4100-Porto, Portugal

Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.

This article has been cited by other articles:

				C. Masclaux-Daubresse, M. Reisdorf-Cren, K. Pageau, M. Lelandais, O. Grandjean, J. Kronenberger, M.-H. Valadier, M. Feraud, T. Jouglet, and A. Suzuki Glutamine Synthetase-Glutamate Synthase Pathway and Glutamate Dehydrogenase Play Distinct Roles in the Sink-Source Nitrogen Cycle in Tobacco Plant Physiology, February 1, 2006; 140(2): 444 - 456. [Abstract] [Full Text] [PDF]

				J. Teixeira, S. Pereira, F. Canovas, and R. Salema Glutamine synthetase of potato (Solanum tuberosum L. cv. Desiree) plants: cell- and organ-specific expression and differential developmental regulation reveal specific roles in nitrogen assimilation and mobilization J. Exp. Bot., February 1, 2005; 56(412): 663 - 671. [Abstract] [Full Text] [PDF]

				P. M. Melo, L. M. Lima, I. M. Santos, H. G. Carvalho, and J. V. Cullimore Expression of the Plastid-Located Glutamine Synthetase of Medicago truncatula. Accumulation of the Precursor in Root Nodules Reveals an in Vivo Control at the Level of Protein Import into Plastids Plant Physiology, May 1, 2003; 132(1): 390 - 399. [Abstract] [Full Text] [PDF]

				I. C. Oliveira, T. Brears, T. J. Knight, A. Clark, and G. M. Coruzzi Overexpression of Cytosolic Glutamine Synthetase. Relation to Nitrogen, Light, and Photorespiration Plant Physiology, July 1, 2002; 129(3): 1170 - 1180. [Abstract] [Full Text] [PDF]

				A. Weber and U.-I. Flugge Interaction of cytosolic and plastidic nitrogen metabolism in plants J. Exp. Bot., April 15, 2002; 53(370): 865 - 874. [Abstract] [Full Text] [PDF]

				A. K. Tobin and T. Yamaya Cellular compartmentation of ammonium assimilation in rice and barley J. Exp. Bot., April 1, 2001; 52(356): 591 - 604. [Abstract] [Full Text] [PDF]

				N. Brugière, F. Dubois, A. M. Limami, M. Lelandais, Y. Roux, R. S. Sangwan, and B. Hirel Glutamine Synthetase in the Phloem Plays a Major Role in Controlling Proline Production PLANT CELL, October 1, 1999; 11(10): 1995 - 2012. [Abstract] [Full Text]

				C. J. Schultz, M. Hsu, B. Miesak, and G. M. Coruzzi Arabidopsis Mutants Define an in Vivo Role for Isoenzymes of Aspartate Aminotransferase in Plant Nitrogen Assimilation Genetics, June 1, 1998; 149(2): 491 - 499. [Abstract] [Full Text] [PDF]