Plant Physiology 100:1846-1851 (1992)
© 1992 American Society of Plant Biologists
Metabolism and Enzymology
Coordinate Regulation of Cytochrome and Alternative Pathway Respiration in Tobacco 1
Greg C. Vanlerberghe and
Lee McIntosh
Michigan State University/Department of Energy Plant Research Laboratory, East Lansing, Michigan 48824,
Biochemistry Department, Michigan State University, East Lansing, Michigan 48824
In suspension cells of NT1 tobacco (Nicotiana tabacum L. cv bright yellow), inhibition of the cytochrome pathway of respiration with antimycin A induced a large increase in the capacity of the alternative pathway over a period of approximately 12 h, as confirmed in both whole cells and isolated mitochondria. The increase in alternative pathway capacity required de novo RNA and protein synthesis and correlated closely with the increase of a 35-kD alternative oxidase protein. When the cytochrome pathway of intact cells was inhibited by antimycin A, respiration proceeded exclusively through the alternative pathway, reached rates significantly higher than before antimycin A addition, and was not stimulated by p-trifluoromethoxycarbonylcyanide (FCCP). When inhibition of the cytochrome pathway was relieved, alternative pathway capacity and the level of the 35-kD alternative oxidase protein declined. Respiration rate also declined and could once again be stimulated by FCCP. These observations show that the capacities of the mitochondrial electron transport pathways can be regulated in a coordinate fashion.
1 This work was supported in part by Department of Energy Grant DE-FG02-90ER20021 (to L.M.) and National Science Foundation Grant D.C.B. 890451B (to L.M.). G.C.V. acknowledges support from a Natural Sciences and Engineering Research Council of Canada Postdoctoral Fellowship.
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