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Metabolism and Enzymology

Evidence for Activation of the Oxidative Pentose Phosphate Pathway during Photosynthetic Assimilation of NO₃^– but Not NH₄⁺ by a Green Alga ¹

Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4

Addition of NO₃^– to N-limited Selenastrum minutum during photosynthesis resulted in an immediate drop in the NADPH/NADP ratio and a slower increase of the NADH/NAD ratio. These changes were accompanied by a rapid decrease in glucose-6-phosphate and increase in 6-phosphogluconate, indicating activation of glucose-6-phosphate dehydrogenase and a role for the oxidation pentose phosphate pathway during photosynthetic NO₃^– assimilation. In contrast, the short-term changes in pyridine nucleotides and metabolites during photosynthetic assimilation of NH₄⁺ were not consistent with a stimulation of the oxidative pentose phosphate pathway.

¹ Supported by the Natural Sciences and Engineering Research Council of Canada (NSERC). H.C.H. was supported by an NSERC International Fellowship.

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