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Metabolism and Enzymology

Substrate-Induced Stability of Glyceraldehyde 3-Phosphate Dehydrogenase from Mung Beans (Vigna radiata L.)

Biotechnology Centre, Indian Agricultural Research Institute, New Delhi-110 012, India, School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi-221 005, India

Time-dependent thermal inactivation of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) present in the extract of mung beans at different periods of germination showed biphasic kinetics in the 12-h germinated seeds but single exponential decay at 24 h of germination. The glyceraldehyde 3-phosphate (G-3-P) concentration in the deproteinated extracts was found to increase with period of germination up to 36 h, parallel to that of GAPDH activity. G-3-P was found to offer protection of the enzyme against thermal inactivation and trypsin digestion. It is suggested that accumulation of G-3-P in germinating mung beans may be of physiological significance and it might offer protection to the enzyme in vivo against thermal inactivation and proteolysis.