|
PLANT PHYSIOLOGY , Vol 101, Issue 1 113-119, Copyright © 1993 by American Society of Plant Biologists
Partial Purification of the Cyanide-Resistant Alternative Oxidase of Skunk Cabbage (Symplocarpus foetidus) Mitochondria
D. A. Berthold and J. N. Siedow
Department of Botany, Duke University, Box 90338, Durham, North Carolina 27708-0338
A partial purification of the cyanide-resistant, alternative oxidase from
skunk cabbage (Symplocarpus foetidus L.) spadix mitochondria is described.
Skunk cabbage mitochondria were solubilized in
N,N-bis-(3-D-glucon-amido-propyl)deoxycholamide and the alternative oxidase
was purified using a batch DEAE-cellulose treatment, followed by
precipitation with Extracti-Gel and chromatography on Sephadex G-200.
Following pooling and concentrating of the most active fractions from the
gel filtration column, a 20- to 30-fold purification of the alternative
oxidase was obtained, with no evidence of contamination by cytochrome c
oxidase (complex IV) or cytochrome c reductase (complex III).
Polyacrylamide gel electrophoresis of the partially purified oxidase showed
major polypeptides at 36 and 29 kD, both of which react with monoclonal
antibodies raised against the Sauromatum guttatum alternative oxidase. The
purified oxidase fraction showed no absorbance in the visible spectral
region, and addition of sodium borohydride induced no absorbance changes in
the ultraviolet region. The purified alternative oxidase catalyzed the
four-electron reduction of oxygen to water in the absence of citrate, but
catalyzed an apparent two-electron reduction of oxygen to hydrogen peroxide
in the presence of 0.7 M citrate.
This article has been cited by other articles:
|
|
|
|
 
Y. Onda, Y. Kato, Y. Abe, T. Ito, M. Morohashi, Y. Ito, M. Ichikawa, K. Matsukawa, Y. Kakizaki, H. Koiwa, et al.
Functional Coexpression of the Mitochondrial Alternative Oxidase and Uncoupling Protein Underlies Thermoregulation in the Thermogenic Florets of Skunk Cabbage
Plant Physiology,
February 1, 2008;
146(2):
636 - 645.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kern, F. S. Hartner, M. Freigassner, J. Spielhofer, C. Rumpf, L. Leitner, K.-U. Frohlich, and A. Glieder
Pichia pastoris 'just in time' alternative respiration
Microbiology,
April 1, 2007;
153(4):
1250 - 1260.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ito, T. Ito, Y. Onda, and M. Uemura
Temperature-Triggered Periodical Thermogenic Oscillations in Skunk Cabbage (Symplocarpus foetidus)
Plant Cell Physiol.,
March 15, 2004;
45(3):
257 - 264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. van Lis, A. Atteia, G. Mendoza-Hernandez, and D. Gonzalez-Halphen
Identification of Novel Mitochondrial Protein Components of Chlamydomonas reinhardtii. A Proteomic Approach
Plant Physiology,
May 1, 2003;
132(1):
318 - 330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Berthold, P. Stenmark, and P. Nordlund
Screening for functional expression and overexpression of a family of diiron-containing interfacial membrane proteins using the univector recombination system
Protein Sci.,
January 1, 2003;
12(1):
124 - 134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Berthold, N. Voevodskaya, P. Stenmark, A. Graslund, and P. Nordlund
EPR Studies of the Mitochondrial Alternative Oxidase. EVIDENCE FOR A DIIRON CARBOXYLATE CENTER
J. Biol. Chem.,
November 8, 2002;
277(46):
43608 - 43614.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-K. Huh and S.-O. Kang
Molecular Cloning and Functional Expression of Alternative Oxidase from Candida albicans
J. Bacteriol.,
July 1, 1999;
181(13):
4098 - 4102.
[Abstract]
[Full Text]
|
|
|
|
|
