Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luinenburg, I.
Right arrow Articles by Coleman, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luinenburg, I.
Right arrow Articles by Coleman, J. R.
Agricola
Right arrow Articles by Luinenburg, I.
Right arrow Articles by Coleman, J. R.

PLANT PHYSIOLOGY , Vol 101, Issue 1 121-126, Copyright © 1993 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

Expression of Escherichia coli Phosphoenolpyruvate Carboxylase in a Cyanobacterium (Functional Complementation of Synechococcus PCC 7942 ppc)

I. Luinenburg and J. R. Coleman
Department of Botany, University of Toronto, Toronto, Ontario, Canada M5S 3B2

The gene (ppc) coding for phosphoenolpyruvate carboxylase (PEPCase) in the cyanobacterium Synechococcus PCC 7942 has been inactivated via insertional mutagenesis while being functionally complemented by Escherichia coli ppc. Cyanobacterial cells functionally complemented by E. coli ppc showed decreased PEPCase activity in crude cell lysates and detergent-permeabilized whole cell assays. Decreased rates of growth, reduced levels of chlorophyll a, and decreased photosynthetic O2 evolution capacity per cell when compared to wild-type cyanobacterial cells were also observed. Phycobiliprotein levels were not affected. The results are discussed in terms of the impact of reduced PEPCase activity on cyanobacterial cell metabolism and the regulatory properties of the E. coli gene product.


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
M.-D. Deng and J. R. Coleman
Ethanol Synthesis by Genetic Engineering in Cyanobacteria
Appl. Envir. Microbiol., February 1, 1999; 65(2): 523 - 528.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1993 by the American Society of Plant Biologists