Plant Physiol. Drug Metab Dispos
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PLANT PHYSIOLOGY , Vol 101, Issue 1 237-243, Copyright © 1993 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

Localization of Branching Enzyme in Potato Tuber Cells with the Use of Immunoelectron Microscopy

A. M. Kram, G. T. Oostergetel and EFJ. van Bruggen
BIOSON Research Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

Potato branching enzyme, a key enzyme in the biosynthesis of starch, was localized in amyloplasts in starch-storage cells of potato (Solanum tuberosum L.) with the use of immunogold electron microscopy. Branching enzyme was found in the amyloplast stroma, concentrated at the interface of the stroma and the surface of the starch granule. ADP-glucose pyrophosphorylase, a key regulatory enzyme in starch synthesis, was localized for comparison to exclude possible artifacts. ADP-glucose pyrophosphorylase, in contrast with branching enzyme, proved to be evenly distributed throughout the stroma. Branching enzyme also appears to be present in a membrane-bounded inclusion body in the stroma, whereas ADP-glucose pyrophosphorylase is not. The presence of branching enzyme predominantly at the surface of the starch granule indicates that branching takes place at that surface and not throughout the amyloplast stroma.


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A. Viksø-Nielsen, A. Blennow, T. Hamborg Nielsen, and B. Lindberg Møller
Phosphorylated alpha (1right-arrow4)Glucans as Substrate for Potato Starch-Branching Enzyme I
Plant Physiology, July 1, 1998; 117(3): 869 - 875.
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Copyright © 1993 by the American Society of Plant Biologists