|
PLANT PHYSIOLOGY , Vol 101, Issue 1 267-276, Copyright © 1993 by American Society of Plant Biologists
|
CELL BIOLOGY AND SIGNAL TRANSDUCTION |
Lipids, Proteins, and Structure of Seed Oil Bodies from Diverse Species
JTC. Tzen, Yz. Cao, P. Laurent, C. Ratnayake and AHC. Huang
Department of Botany and Plant Sciences, University of California, Riverside, California 92521-0124
Oil bodies isolated from the mature seeds of rape (Brassica napus L.),
mustard (Brassica juncea L.), cotton (Gossypium hirsutum L.), flax (Linus
usitatis simum), maize (Zea mays L.), peanut (Arachis hypogaea L.), and
sesame (Sesamum indicum L.) had average diameters that were different but
within a narrow range (0.6-2.0 [mu]m), as measured from electron
micrographs of serial sections. Their contents of triacylglycerols (TAG),
phospholipids, and proteins (oleosins) were correlated with their sizes.
The correlation fits a formula that describes a spherical particle
surrounded by a shell of a monolayer of phospholipids embedded with
oleosins. Oil bodies from the various species contained substantial amounts
of the uncommon negatively charged phosphatidylserine and
phosphatidylinositol, as well as small amounts of free fatty acids. These
acidic lipids are assumed to interact with the basic amino acid residues of
the oleosins on the surface of the phospholipid layer. Isoelectrofocusing
revealed that the oil bodies from the various species had an isoelectric
point of 5.7 to 6.6 and thus possessed a negatively charged surface at
neutral pH. We conclude that seed oil bodies from diverse species are very
similar in structure. In rapeseed during maturation, TAG and oleosins
accumulated concomitantly. TAG-synthesizing acyltransferase activities
appeared at an earlier stage and peaked during the active period of TAG
accumulation. The concomitant accumulation of TAG and oleosins is similar
to that reported earlier for maize and soybean, and the finding has an
implication for the mode of oil body synthesis during seed maturation.
This article has been cited by other articles:
|
|
|
|
 
K. Lardizabal, R. Effertz, C. Levering, J. Mai, M.C. Pedroso, T. Jury, E. Aasen, K. Gruys, and K. Bennett
Expression of Umbelopsis ramanniana DGAT2A in Seed Increases Oil in Soybean
Plant Physiology,
September 1, 2008;
148(1):
89 - 96.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M.P. Siloto, K. Findlay, A. Lopez-Villalobos, E. C. Yeung, C. L. Nykiforuk, and M. M. Moloney
The Accumulation of Oleosins Determines the Size of Seed Oilbodies in Arabidopsis
PLANT CELL,
August 1, 2006;
18(8):
1961 - 1974.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. I. MANTESE, D. MEDAN, and A. J. HALL
Achene Structure, Development and Lipid Accumulation in Sunflower Cultivars Differing in Oil Content at Maturity
Ann. Bot.,
June 1, 2006;
97(6):
999 - 1010.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Waltermann and A. Steinbuchel
Neutral Lipid Bodies in Prokaryotes: Recent Insights into Structure, Formation, and Relationship to Eukaryotic Lipid Depots
J. Bacteriol.,
June 1, 2005;
187(11):
3607 - 3619.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. S. Anil, A. C. Harmon, and K. S. Rao
Temporal Association of Ca2+-Dependent Protein Kinase with Oil Bodies during Seed Development in Santalum album L.: Its Biochemical Characterization and Significance
Plant Cell Physiol.,
April 15, 2003;
44(4):
367 - 376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. W. Hou, M. Y. Lin, M. M. C. Wang, and J. T. C. Tzen
Increase of Viability of Entrapped Cells of Lactobacillus delbrueckii ssp. bulgaricus in Artificial Sesame Oil Emulsions
J Dairy Sci,
February 1, 2003;
86(2):
424 - 428.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Cordova-Tellez and J. S. Burris
Alignment of Lipid Bodies along the Plasma Membrane during the Acquisition of Desiccation Tolerance in Maize Seed
Crop Sci.,
November 1, 2002;
42(6):
1982 - 1988.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. U. Kim, K. Hsieh, C. Ratnayake, and A. H. C. Huang
A Novel Group of Oleosins Is Present Inside the Pollen of Arabidopsis
J. Biol. Chem.,
June 14, 2002;
277(25):
22677 - 22684.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C.F. Chen and J. T.C. Tzen
An in vitro System to Examine the Effective Phospholipids and Structural Domain for Protein Targeting to Seed Oil Bodies
Plant Cell Physiol.,
November 1, 2001;
42(11):
1245 - 1252.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Jako, A. Kumar, Y. Wei, J. Zou, D. L. Barton, E. M. Giblin, P. S. Covello, and D. C. Taylor
Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight
Plant Physiology,
June 1, 2001;
126(2):
861 - 874.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Y. Bih, S. S. H. Wu, C. Ratnayake, L. L. Walling, E. A. Nothnagel, and A. H. C. Huang
The Predominant Protein on the Surface of Maize Pollen Is an Endoxylanase Synthesized by a Tapetum mRNA with a Long 5' Leader
J. Biol. Chem.,
August 6, 1999;
274(32):
22884 - 22894.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. H. Wu, K. A. Platt, C. Ratnayake, T.-W. Wang, J. T. L. Ting, and A. H. C. Huang
Isolation and characterization of neutral-lipid-containing organelles and globuli-filled plastids from Brassica napus tapetum
PNAS,
November 11, 1997;
94(23):
12711 - 12716.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-J. Lin, S. S.K. Tai, C.-C. Peng, and J. T.C. Tzen
Steroleosin, a Sterol-Binding Dehydrogenase in Seed Oil Bodies
Plant Physiology,
April 1, 2002;
128(4):
1200 - 1211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
