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PLANT PHYSIOLOGY , Vol 101, Issue 1 329-332, Copyright © 1993 by American Society of Plant Biologists

RAPID COMMUNICATIONS

Partial Sequence Analysis of 130 Randomly Selected Maize cDNA Clones

C. S. Keith, D. O. Hoang, B. M. Barrett, B. Feigelman, M. C. Nelson, H. Thai and C. Baysdorfer
Department of Biological Sciences, California State University, Hayward, California 94542

As part of a project to identify novel maize (Zea mays L. cv B73) genes functionally, we have partially sequenced 130 randomly selected clones from a maize leaf cDNA library. Data base comparisons revealed seven previously sequenced maize cDNAs and 18 cDNAs with sequence similarity to related maize genes or to genes from other organisms. One hundred five cDNAs show little or no similarity to previously sequenced genes. Our results also establish the suitability of this library for large-scale sequencing in terms of its large insert size, proper insert orientation, and low duplication rate.


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