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PLANT PHYSIOLOGY , Vol 101, Issue 2 441-450, Copyright © 1993 by American Society of Plant Biologists
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MOLECULAR BIOLOGY AND GENE REGULATION |
An Arabidopsis thaliana Lipoxygenase Gene Can Be Induced by Pathogens, Abscisic Acid, and Methyl Jasmonate
M. A. Melan, X. Dong, M. E. Endara, K. R. Davis, F. M. Ausubel and T. K. Peterman
Department of Biological Sciences, Wellesley College, Wellesley, Massachusetts 02181 (M.A.M., M.E.E., T.K.P.)
We isolated and characterized a 2.8-kb, full-length, Arabidopsis thaliana
cDNA clone encoding a lipoxygenase. DNA sequence analysis showed that the
deduced amino acid sequence of the Arabidopsis protein is 72 to 78% similar
to that of legume seed lipoxygenases. DNA blot analysis indicated that
Arabidopsis contains a single gene, LOX1, with appreciable homology to the
cDNA clone. RNA blot analysis showed that the LOX1 gene is expressed in
Arabidopsis leaves, roots, inflorescences, and young seedlings. LOX1
expression levels were highest in roots and young seedlings. In mature
plants, LOX1 mRNA levels increased upon treatment with the stress-related
hormones abscisic acid and methyl jasmonate and remained high for at least
96 h. Expression of the LOX1 gene was examined following infiltration of
leaves with virulent (Psm ES4326) and avirulent (Pst MM1065) strains of
Pseudomonas syringae. LOX1 mRNA levels were induced approximately 6-fold by
both virulent and avirulent strains; however, the response to avirulent
strains was much more rapid. Infiltration of leaves with Pst MM1065
resulted in maximal induction within 12 h, whereas maximal induction by Psm
ES4326 did not occur until 48 h. When a cloned avr gene, avrRpt2, was
transferred to Psm ES4326, LOX1 mRNA accumulated in a pattern similar to
that observed for the avirulent strain Pst MM1065.
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