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PLANT PHYSIOLOGY , Vol 102, Issue 1 45-51, Copyright © 1993 by American Society of Plant Biologists

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Effect of Intracellular Glutathione Level on the Production of 6-Methoxymellein in Cultured Carrot (Daucus carota) Cells

Zj. Guo, S. Nakagawara, K. Sumitani and Y. Ohta
Yamazaki Laboratory, PCC Technology Inc., 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618, Japan

To produce phytoalexin, 6-methoxymellein (6-MM) was induced in suspension cultures of carrot (Daucus carota) by buthionine sulfoximine (BSO) and CuCl2. Addition of BSO (a specific inhibitor of glutathione [GSH] synthesis) to the cultures lowered the cellular GSH levels. This depletion of GSH was BSO-concentration dependent, and the extent of 6-MM accumulation was dependent on the GSH depletion. The accumulation of 6-MM induced by BSO was suppressed by exogenous GSH. Exogenous H2O2 stimulated the production of 6-MM when added 1 d after BSO treatment, whereas H2O2 added at time zero or on the 4th d of BSO treatment did not. Moreover, a synergistic effect of simultaneous addition of BSO and CuCl2 was observed. These results suggest that active oxygen species may be involved in the triggering of 6-MM synthesis.


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H. Vanacker, T. L.W. Carver, and C. H. Foyer
Pathogen-Induced Changes in the Antioxidant Status of the Apoplast in Barley Leaves
Plant Physiology, July 1, 1998; 117(3): 1103 - 1114.
[Abstract] [Full Text]


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Copyright © 1993 by the American Society of Plant Biologists