PLANT PHYSIOLOGY , Vol 102, Issue 3 1027-1031, Copyright © 1993 by American Society of Plant Biologists
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DEVELOPMENT AND GROWTH REGULATION |
Tissue Printing as a Tool for Observing Immunological and Protein Profiles in Young and Mature Celery Petioles
R. Taylor, G. Inamine and J. D. Anderson
Weed Science Laboratory, Beltsville Agriculture Research Center West, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705
Tissue printing onto membranes such as nitrocellulose is a technique
employed to study the localization of proteins, nucleic acids, and soluble
metabolites from freshly cut tissue slices. We probed tissue prints of
young and mature celery (Apium graveolens) petioles with antibodies raised
against two proteins, spinach ribulose-1,5-bisphosphate carboxylase and
tomato fruit catalase. The purposes of this study were to determine if
these proteins are developmentally regulated and to determine if the
patterns and intensities of cross-reactivity of antibodies on tissue blots
corresponded only to the presence of specific epitopes or was related to
the amount of protein present in any given area on the tissue prints.
Different and distinct cross-reactivity patterns were observed with each of
the two antibodies used. Tissue prints from young and mature tissues also
showed differences in antibody cross-reactivity. Comparison of Coomassie
blue staining patterns with antibody reactivity patterns showed that there
is little relationship between tissue protein concentration and antibody
reactivity.
