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PLANT PHYSIOLOGY , Vol 102, Issue 4 1139-1146, Copyright © 1993 by American Society of Plant Biologists
Calcium Retrieval from Vacuolar Pools (Characterization of a Vacuolar Calcium Channel)
A. Gelli and E. Blumwald
Centre for Plant Biotechnology, Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 3B2, Canada
Voltage patch-clamp experiments at the whole-vacuole and single-channel
levels were employed to study the retrieval of Ca2+ from vacuoles into the
cytoplasm in sugar beet cell (Beta vulgaris L.) suspension cultures.
Channels allowing the movement of Ca2+ out of the vacuole were identified
at physiological conditions of pH, vacuolar membrane potential, and
vacuole/cytoplasm Ca2+ concentrations. The operation of the channel was
voltage dependent and inositol-1,4,5-triphosphate insensitive and displayed
high selectivity for Ca2+ ions. These channels bear similarities to the
dihydropyridine-sensitive L-type Ca2+ channels from animal cells. Bay
K-8644, an agonist, increased the frequency of channel openings, whereas
nifedipine, an antagonist, reduced the channel activity. Both effects were
elicited only from the vacuolar side of the channel. Channel activities
were also inhibited by verapamil, La3+, and cytoplasmic Ca2+ concentrations
higher than 1 x 10-6 M. The modulation of the channel currents by
cytoplasmic Ca2+ would suggest the role of these channels in triggering the
initiation of signal transduction processes in plant cells.
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