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PLANT PHYSIOLOGY , Vol 103, Issue 1 97-103, Copyright © 1993 by American Society of Plant Biologists
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MOLECULAR BIOLOGY AND GENE REGULATION |
Identification of Methyl Jasmonate and Salicylic Acid Response Elements from the Nopaline Synthase (nos) Promoter
S. R. Kim, Y. Kim and G. An
Institute of Biological Chemistry (S.-R.K., Y.K., G.A.) and Department of Genetics and Cell Biology (S.-R. K., G.A.), Washington State University, Pullman, Washington 99164
Transgenic tobacco plants carrying a fusion between the nopaline synthase
(nos) promoter and chloramphenicol acetyltransferase (CAT) reporter gene
(cat) were studied for their inducibility by salicylic acid (SA) or methyl
jasmonate (MJ) treatments. Either chemical significantly increased CAT
activity to a level much higher than that achieved by wounding. Northern
blot analysis showed a corresponding increase in mRNA levels. After 20 h of
induction of flowering plants, the response to MJ treatment was weaker in
old leaves compared with young leaves, whereas the SA response was stronger
in old leaves. Kinetic experiments showed that the SA response was much
faster than the MJ response, suggesting that the induction mechanism of the
nos promoter by these chemicals may differ. Deletion analysis showed that
both SA and MJ responses require the DNA sequence between -119 and -112
from the transcription initiation site. This region contains the hexamer
sequence (TGACGT) that has been found to be an important regulatory element
for several promoters. The MJ response was also reduced by deletions of the
CAAT box region or the sequence between -112 and -101, whereas the SA
response was not significantly affected by these deletions. This suggests
that the nos upstream region containing the hexamer motif is essential for
the SA or MJ response and that the CAAT box region and the sequence
immediately downstream from the hexamer motif are required for maximum
induction by MJ.
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