Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (68)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rasi-Caldognov, F.
Right arrow Articles by De Michelis, M. I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rasi-Caldognov, F.
Right arrow Articles by De Michelis, M. I.
Agricola
Right arrow Articles by Rasi-Caldognov, F.
Right arrow Articles by De Michelis, M. I.

PLANT PHYSIOLOGY , Vol 103, Issue 2 391-398, Copyright © 1993 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

Controlled Proteolysis Mimics the Effect of Fusicoccin on the Plasma Membrane H+-ATPase

F. Rasi-Caldognov, M. C. Pugliarello, C. Olivari and M. I. De Michelis
Centro di Studio del Consiglio Nazionale delle Ricerche per la Biologia Cellulare e Molecolare delle Piante, Dipartimento di Biologia, Universita di Milano, via G. Celoria 26, 20133 Milano, Italy (F.R.-C., M.C.P., C.O.)

We analyzed the effects of controlled treatments with trypsin of plasma membrane (PM) isolated from radish (Raphanus sativus L.) seedlings on the activity of the PM H+-ATPase, and we compared them with those of fusicoccin (FC). Mild treatments of the PM with trypsin, which led to a decrease of the molecular mass of the peptide of about 10 kD, markedly increased the H+-ATPase activity. The effect strongly increased with the increase of pH of the assay medium from 6.1 to 7.5, so the pH optimum of the enzyme activity shifted from 6.8 in untreated PM to 7.1 in trypsin-treated PM. The proteolytic treatment activated only the portion of PM H+-ATPase activity that is stable to preincubation in assay medium in the absence of ATP and determined a strong increase of Vmax and a less marked decrease of the apparent Km for Mg-ATP. All of these effects were very similar to those determined by FC, which activated the PM H+-ATPase without promoting its proteolytic cleavage. FC did not further activate the H+-ATPase activity of trypsin-treated PM under conditions in which the FC receptor was protected from the attack of trypsin. Conversely, trypsin treatment had little effect on the PM H+-ATPase preactivated with FC. Moreover, the activity of the PM H+-ATPase preactivated with FC was not further activated by Iysolecithin. These results indicate that the modification of the PM H+-ATPase of higher plants triggered by the FC-receptor complex hinders the inhibitory interaction of the regulatory C-terminal domain with the active site.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
X.-C. Yu, M.-J. Li, G.-F. Gao, H.-Z. Feng, X.-Q. Geng, C.-C. Peng, S.-Y. Zhu, X.-J. Wang, Y.-Y. Shen, and D.-P. Zhang
Abscisic Acid Stimulates a Calcium-Dependent Protein Kinase in Grape Berry
Plant Physiology, February 1, 2006; 140(2): 558 - 579.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Alsterfjord, P. C. Sehnke, A. Arkell, H. Larsson, F. Svennelid, M. Rosenquist, R. J. Ferl, M. Sommarin, and C. Larsson
Plasma Membrane H+-ATPase and 14-3-3 Isoforms of Arabidopsis Leaves: Evidence for Isoform Specificity in the 14-3-3/H+-ATPase Interaction
Plant Cell Physiol., September 15, 2004; 45(9): 1202 - 1210.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kinoshita and K.-i. Shimazaki
Biochemical Evidence for the Requirement of 14-3-3 Protein Binding in Activation of the Guard-cell Plasma Membrane H+-ATPase by Blue Light
Plant Cell Physiol., November 15, 2002; 43(11): 1359 - 1365.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kinoshita and K.-i. Shimazaki
Analysis of the Phosphorylation Level in Guard-Cell Plasma Membrane H+-ATPase in Response to Fusicoccin
Plant Cell Physiol., April 1, 2001; 42(4): 424 - 432.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Dell'Orto, S. Santi, P. De Nisi, S. Cesco, Z. Varanini, G. Zocchi, and R. Pinton
Development of Fe-deficiency responses in cucumber (Cucumis sativus L.) roots: involvement of plasma membrane H+-ATPase activity
J. Exp. Bot., April 1, 2000; 51(345): 695 - 701.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Camoni, V. Iori, M. Marra, and P. Aducci
Phosphorylation-dependent Interaction between Plant Plasma Membrane H+-ATPase and 14-3-3 Proteins
J. Biol. Chem., March 31, 2000; 275(14): 9919 - 9923.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Olivari, C. Albumi, M. C. Pugliarello, and M. I. De Michelis
Phenylarsine Oxide Inhibits the Fusicoccin-Induced Activation of Plasma Membrane H+-ATPase
Plant Physiology, February 1, 2000; 122(2): 463 - 470.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
A. Olsson, F. Svennelid, B. Ek, M. Sommarin, and C. Larsson
A Phosphothreonine Residue at the C-Terminal End of the Plasma Membrane H+-ATPase Is Protected by Fusicoccin-Induced 14-3-3 Binding
Plant Physiology, October 1, 1998; 118(2): 551 - 555.
[Abstract] [Full Text]

Home page
 GeneticsHome page
J. C. Young, N. D. DeWitt, and M. R. Sussman
A Transgene Encoding a Plasma Membrane H+-ATPase That Confers Acid Resistance in Arabidopsis thaliana Seedlings
Genetics, June 1, 1998; 149(2): 501 - 507.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. R. Fullone, S. Visconti, M. Marra, V. Fogliano, and P. Aducci
Fusicoccin Effect on the in Vitro Interaction between Plant 14-3-3 Proteins and Plasma Membrane H+-ATPase
J. Biol. Chem., March 27, 1998; 273(13): 7698 - 7702.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Olivari, C. Meanti, M. I. De Michelis, and F. Rasi-Caldogno
Fusicoccin Binding to Its Plasma Membrane Receptor and the Activation of the Plasma Membrane H+-ATPase . IV. Fusicoccin Induces the Association between the Plasma Membrane H+-ATPase and the Fusicoccin Receptor
Plant Physiology, February 1, 1998; 116(2): 529 - 537.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1993 by the American Society of Plant Biologists