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PLANT PHYSIOLOGY , Vol 103, Issue 2 441-448, Copyright © 1993 by American Society of Plant Biologists
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MOLECULAR BIOLOGY AND GENE REGULATION |
Long-Term Anoxia Tolerance (Multi-Level Regulation of Gene Expression in the Amphibious Plant Acorus calamus L.)
M. Bucher and C. Kuhlemeier
Institute of Plant Physiology, University of Berne, Altenbergrain 21, CH-3013 Berne, Switzerland
Acorus calamus is a monocotyledonous wetland plant that can withstand
extremely long periods of anoxia. We have investigated the expression of
genes coding for pyruvate decarboxylase (Pdc), alcohol dehydrogenase (Adh),
and fructose-1,6-bisphosphate aldolase (Ald) during periods of anoxia
ranging from 2 h to 2 months. Upon anoxic incubation, Pdc mRNA levels peak
at 6 h, followed by Adh and Ald, which peak at 12 and 72 h, respectively.
Subsequently, the mRNA levels of all three genes decline within days to low
levels. In contrast, alcohol dehydrogenase (ADH) protein levels increase
steadily for at least a week and then remain constant. Native gel
electrophoresis demonstrates the presence of two sets of ADH isozymes, one
present constitutively, the other enhanced during anoxia. Translation
initiation factor 4A protein levels, used as a control, remain constant
during 2 months of anoxia. The results suggest that A. calamus has
developed a complex anaerobic response consisting of differential
regulation of transcription, translation, and posttranslational processes.
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