|
PLANT PHYSIOLOGY , Vol 103, Issue 3 771-781, Copyright © 1993 by American Society of Plant Biologists
|
MOLECULAR BIOLOGY AND GENE REGULATION |
Osmoregulation of a Pyrroline-5-Carboxylate Reductase Gene in Arabidopsis thaliana
N. Verbruggen, R. Villarroel and M. Van Montagu
Laboratorium voor Genetica, Universiteit Gent, B-9000 Gent, Belgium
In Arabidopsis thaliana (L.) Heynh. proline can account for up to 20% of
the free amino acid pool after salt stress. Proline accumulation occurs in
plants mainly by de novo synthesis from glutamate. The last step of the
proline biosynthetic pathway is catalyzed by pyrroline-5-carboxylate (P5C)
reductase. A gene (AT-P5C1) encoding this enzyme in A. thaliana has been
cloned and sequenced. Expression of AT-P5C1 in Escherichia coli resulted in
the complementation of a proC mutant to prototrophy. A comparison of the
AT-P5C1 primary and secondary structures with those of six P5C reductases
of other organisms is presented. With the exception of several functionally
important amino acid residues, little conservation in the primary structure
is seen; much greater similarity exists in the putative secondary
structure. The AT-P5C1 protein is probably cytosolic. Under normal growth
conditions, the P5C reductase mRNA level was significantly higher in roots
and ripening seeds than in green tissue. A salt treatment of A. thaliana
plants resulted in a 5-fold induction of the AT-P5C1 transcript, suggesting
osmoregulation of the AT-P5C1 promoter region. Moreover, a time-course
experiment indicated that this induction precedes proline accumulation.
This article has been cited by other articles:
|
|
|
|
 
E. Parre, M. A. Ghars, A.-S. Leprince, L. Thiery, D. Lefebvre, M. Bordenave, L. Richard, C. Mazars, C. Abdelly, and A. Savoure
Calcium Signaling via Phospholipase C Is Essential for Proline Accumulation upon Ionic But Not Nonionic Hyperosmotic Stresses in Arabidopsis
Plant Physiology,
May 1, 2007;
144(1):
503 - 512.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Goyer, T. L. Johnson, L. J. Olsen, E. Collakova, Y. Shachar-Hill, D. Rhodes, and A. D. Hanson
Characterization and Metabolic Function of a Peroxisomal Sarcosine and Pipecolate Oxidase from Arabidopsis
J. Biol. Chem.,
April 23, 2004;
279(17):
16947 - 16953.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Thiery, A.-S. Leprince, D. Lefebvre, M. A. Ghars, E. Debarbieux, and A. Savoure
Phospholipase D Is a Negative Regulator of Proline Biosynthesis in Arabidopsis thaliana
J. Biol. Chem.,
April 9, 2004;
279(15):
14812 - 14818.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Knight, C. M. Duckett, J. A. Sullivan, A. R. Walker, and J. C. Gray
Tissue-Specific, Light-Regulated and Plastid-Regulated Expression of the Single-Copy Nuclear Gene Encoding the Chloroplast Rieske FeS Protein of Arabidopsis thaliana
Plant Cell Physiol.,
May 15, 2002;
43(5):
522 - 531.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Murahama, T. Yoshida, F. Hayashi, T. Ichino, Y. Sanada, and K. Wada
Purification and Characterization of {Delta}1-Pyrroline-5-Carboxylate Reductase Isoenzymes, Indicating Differential Distribution in Spinach (Spinacia oleracea L.) Leaves
Plant Cell Physiol.,
July 1, 2001;
42(7):
742 - 750.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hellmann, D. Funck, D. Rentsch, and W. B. Frommer
Hypersensitivity of an Arabidopsis Sugar Signaling Mutant toward Exogenous Proline Application
Plant Physiology,
June 1, 2000;
123(2):
779 - 789.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Hellmann, D. Funck, D. Rentsch, and W. B. Frommer
Hypersensitivity of an Arabidopsis Sugar Signaling Mutant toward Exogenous Proline Application
Plant Physiology,
February 1, 2000;
122(2):
357 - 368.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. H. Roosens, R. Willem, Y. Li, I. Verbruggen, M. Biesemans, and M. Jacobs
Proline Metabolism in the Wild-Type and in a Salt-Tolerant Mutant of Nicotiana plumbaginifolia Studied by 13C-Nuclear Magnetic Resonance Imaging
Plant Physiology,
December 1, 1999;
121(4):
1281 - 1290.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. H. Lee, M. Van Montagu, and N. Verbruggen
A highly conserved kinase is an essential component for stress tolerance in yeast and plant cells
PNAS,
May 11, 1999;
96(10):
5873 - 5877.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. E. Verslues and R. E. Sharp
Proline Accumulation in Maize (Zea mays L.) Primary Roots at Low Water Potentials. II. Metabolic Source of Increased Proline Deposition in the Elongation Zone
Plant Physiology,
April 1, 1999;
119(4):
1349 - 1360.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Fujita, A. Maggio, M. Garcia-Rios, R. A. Bressan, and L. N. Csonka
Comparative Analysis of the Regulation of Expression and Structures of Two Evolutionarily Divergent Genes for Delta 1-Pyrroline-5-Carboxylate Synthetase from Tomato
Plant Physiology,
October 1, 1998;
118(2):
661 - 674.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. A. Wright and D. F. Voytas
Potential Retroviruses in Plants: Tat1 Is Related to a Group of Arabidopsis thaliana Ty3/gypsy Retrotransposons That Encode Envelope-Like Proteins
Genetics,
June 1, 1998;
149(2):
703 - 715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. H.C.J. Roosens, T. T. Thu, H. M. Iskandar, and M. Jacobs
Isolation of the Ornithine-delta -Aminotransferase cDNA and Effect of Salt Stress on Its Expression in Arabidopsis thaliana
Plant Physiology,
May 1, 1998;
117(1):
263 - 271.
[Abstract]
[Full Text]
|
|
|
|
|
