PLANT PHYSIOLOGY , Vol 103, Issue 3 979-985, Copyright © 1993 by American Society of Plant Biologists

DEVELOPMENT AND GROWTH REGULATION

Pollen Tube and Root-Hair Tip Growth Is Disrupted in a Mutant of Arabidopsis thaliana

J. Schiefelbein, M. Galway, J. Masucci and S. Ford
Department of Biology, University of Michigan, Ann Arbor, Michigan 48109

The expansion of both root hairs and pollen tubes occurs by a process known as tip growth. In this report, an Arabidopsis thaliana mutant (tip1) is described that displays defects in both root-hair and pollen-tube growth. The root hairs of the tip1 mutant plants are shorter than those of the wild-type plants and branched at their base. The tip1 pollen-tube growth defect was identified by the aberrant segregation ratio of phenotypically normal to mutant seeds in siliques from self-pollinated, heterozygous plants. Homozygous mutant seeds are not randomly distributed in the siliques, comprising only 14.4% of the total seeds, 5.3% of the seeds from the bottom half, and 2.2% of the seeds from the bottom quarter of the heterozygous siliques. Studies of pollen-tube growth in vivo showed that mutant pollen tubes grow more slowly than wild-type pollen through the transmitting tissue of wild-type flowers. Cosegregation studies indicate that the root-hair and pollen-tube defects are caused by the same genetic lesion. Based on these findings, the TIP1 gene is likely to encode a product involved in a fundamental aspect of tip growth in plant cells.

This article has been cited by other articles:

				A. L. Szumlanski and E. Nielsen The Rab GTPase RabA4d Regulates Pollen Tube Tip Growth in Arabidopsis thaliana PLANT CELL, February 1, 2009; 21(2): 526 - 544. [Abstract] [Full Text] [PDF]

				S. Guimil and C. Dunand Cell growth and differentiation in Arabidopsis epidermal cells J. Exp. Bot., November 1, 2007; 58(14): 3829 - 3840. [Abstract] [Full Text] [PDF]

				P. A. Hemsley, A. C. Kemp, and C. S. Grierson The TIP GROWTH DEFECTIVE1 S-Acyl Transferase Regulates Plant Cell Growth in Arabidopsis PLANT CELL, September 1, 2005; 17(9): 2554 - 2563. [Abstract] [Full Text] [PDF]

				C. Ringli, N. Baumberger, and B. Keller The Arabidopsis Root Hair Mutants der2-der9 are Affected at Different Stages of Root Hair Development Plant Cell Physiol., July 1, 2005; 46(7): 1046 - 1053. [Abstract] [Full Text] [PDF]

				M. L. Tansengco, H. Imaizumi-Anraku, M. Yoshikawa, S. Takagi, M. Kawaguchi, M. Hayashi, and Y. Murooka Pollen Development and Tube Growth are Affected in the Symbiotic Mutant of Lotus japonicus, crinkle Plant Cell Physiol., May 15, 2004; 45(5): 511 - 520. [Abstract] [Full Text] [PDF]

				M. Muller and W. Schmidt Environmentally Induced Plasticity of Root Hair Development in Arabidopsis Plant Physiology, January 1, 2004; 134(1): 409 - 419. [Abstract] [Full Text] [PDF]

				C. Ringli, N. Baumberger, A. Diet, B. Frey, and B. Keller ACTIN2 Is Essential for Bulge Site Selection and Tip Growth during Root Hair Development of Arabidopsis Plant Physiology, August 1, 2002; 129(4): 1464 - 1472. [Abstract] [Full Text] [PDF]

				M. Bucher, S. Brunner, P. Zimmermann, G. I. Zardi, N. Amrhein, L. Willmitzer, and J. W. Riesmeier The Expression of an Extensin-Like Protein Correlates with Cellular Tip Growth in Tomato Plant Physiology, March 1, 2002; 128(3): 911 - 923. [Full Text] [PDF]

				K. Mouline, A.-A. Very, F. Gaymard, J. Boucherez, G. Pilot, M. Devic, D. Bouchez, J.-B. Thibaud, and H. Sentenac Pollen tube development and competitive ability are impaired by disruption of a Shaker K+ channel in Arabidopsis Genes & Dev., February 1, 2002; 16(3): 339 - 350. [Abstract] [Full Text] [PDF]

				N. Baumberger, C. Ringli, and B. Keller The chimeric leucine-rich repeat/extensin cell wall protein LRX1 is required for root hair morphogenesis in Arabidopsis thaliana Genes & Dev., May 1, 2001; 15(9): 1128 - 1139. [Abstract] [Full Text]

				S. Rigas, G. Debrosses, K. Haralampidis, F. Vicente-Agullo, K. A. Feldmann, A. Grabov, L. Dolan, and P. Hatzopoulos TRH1 Encodes a Potassium Transporter Required for Tip Growth in Arabidopsis Root Hairs PLANT CELL, January 1, 2001; 13(1): 139 - 151. [Abstract] [Full Text]

				J. W. Schiefelbein Constructing a Plant Cell. The Genetic Control of Root Hair Development Plant Physiology, December 1, 2000; 124(4): 1525 - 1531. [Full Text]

				J. S. Parker, A. C. Cavell, L. Dolan, K. Roberts, and C. S. Grierson Genetic Interactions during Root Hair Morphogenesis in Arabidopsis PLANT CELL, October 1, 2000; 12(10): 1961 - 1974. [Abstract] [Full Text]

				S. E. Travers and T. P. Holtsford Differential siring success of Pgi genotypes in Clarkia Unguiculata (Onagraceae) Am. J. Botany, August 1, 2000; 87(8): 1091 - 1098. [Abstract] [Full Text]

				C. Bernhardt and M. L. Tierney Expression of AtPRP3, a Proline-Rich Structural Cell Wall Protein from Arabidopsis, Is Regulated by Cell-Type-Specific Developmental Pathways Involved in Root Hair Formation Plant Physiology, March 1, 2000; 122(3): 705 - 714. [Abstract] [Full Text] [PDF]

				P. E. Grini, A. Schnittger, H. Schwarz, I. Zimmermann, B. Schwab, G. Jürgens, and M. Hülskamp Isolation of Ethyl Methanesulfonate-Induced Gametophytic Mutants in Arabidopsis thaliana by a Segregation Distortion Assay Using the Multimarker Chromosome 1 Genetics, February 1, 1999; 151(2): 849 - 863. [Abstract] [Full Text]

				H Wang, S K Lockwood, M F Hoeltzel, and J W Schiefelbein The ROOT HAIR DEFECTIVE3 gene encodes an evolutionarily conserved protein with GTP-binding motifs and is required for regulated cell enlargement in Arabidopsis. Genes & Dev., March 15, 1997; 11(6): 799 - 811. [Abstract] [PDF]

				M. A. Jones, J.-J. Shen, Y. Fu, H. Li, Z. Yang, and C. S. Grierson The Arabidopsis Rop2 GTPase Is a Positive Regulator of Both Root Hair Initiation and Tip Growth PLANT CELL, April 1, 2002; 14(4): 763 - 776. [Abstract] [Full Text] [PDF]