Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (49)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hetherington, P. R.
Right arrow Articles by Fry, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hetherington, P. R.
Right arrow Articles by Fry, S. C.
Agricola
Right arrow Articles by Hetherington, P. R.
Right arrow Articles by Fry, S. C.

PLANT PHYSIOLOGY , Vol 103, Issue 3 987-992, Copyright © 1993 by American Society of Plant Biologists

DEVELOPMENT AND GROWTH REGULATION

Xyloglucan Endotransglycosylase Activity in Carrot Cell Suspensions during cell Elongation and Somatic Embryogenesis

P. R. Hetherington and S. C. Fry
Centre for Plant Science, Division of Biological Sciences, University of Edinburgh, Daniel Rutherford Building, The King's Buildings, Mayfield Road, Edinburgh, EH9 3JH, United Kingdom

Xyloglucan endotransglycosylase (XET) has been proposed to contribute to cell elongation through wall loosening. To explore this relationship further, we assayed this enzyme activity in suspensions of carrot (Daucus carota L.) cells exhibiting various rates of cell elongation. In one cell line, elongation was induced by dilution into dichlorophenoxyacetic acid (2,4-D)-free medium. During this elongation, 93% of the XET activity was found in the culture medium; in nonelongating controls, by contrast, 68% was found in the cell extracts even though the specific activity of these extracts was lower than in the elongating cells. By far the highest rates of XET secretion per cell were in the elongating cells. A second cell line was induced to undergo somatic embryogenesis by dilution into 2,4-D-free medium. During the first 6 d, numerous globular embryoids composed of small, isodiametric cells were formed in the absence of cell elongation; extracellular XET activity was almost undetectable, and intracellular specific activity markedly declined. After 6 d, heart, torpedo, and cotyledonary embryoids began to appear (i.e. cell elongation resumed); the intracellular specific activity of XET rose rapidly and >80% of the XET activity accumulated in the medium. Thus, nonexpanding cell suspensions (whether or not they were rapidly dividing) produced and secreted less XET activity than did expanding cells. We propose that a XET molecule has an ephemeral wall-loosening role while it passes through the load-bearing layer of the wall on its way from the protoplast into the culture medium.


This article has been cited by other articles:


Home page
 J Exp BotHome page
A. Maris, D. Suslov, S. C. Fry, J.-P. Verbelen, and K. Vissenberg
Enzymic characterization of two recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis and their effect on root growth and cell wall extension
J. Exp. Bot., September 1, 2009; 60(13): 3959 - 3972.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
V. S. T. Van Sandt, D. Suslov, J.-P. Verbelen, and K. Vissenberg
Xyloglucan Endotransglucosylase Activity Loosens a Plant Cell Wall
Ann. Bot., December 1, 2007; 100(7): 1467 - 1473.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Vissenberg, S. C. Fry, M. Pauly, H. Hofte, and J.-P. Verbelen
XTH acts at the microfibril-matrix interface during cell elongation
J. Exp. Bot., February 1, 2005; 56(412): 673 - 683.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. K. C. Rose, J. Braam, S. C. Fry, and K. Nishitani
The XTH Family of Enzymes Involved in Xyloglucan Endotransglucosylation and Endohydrolysis: Current Perspectives and a New Unifying Nomenclature
Plant Cell Physiol., December 15, 2002; 43(12): 1421 - 1435.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Wu and D. J. Cosgrove
Adaptation of roots to low water potentials by changes in cell wall extensibility and cell wall proteins
J. Exp. Bot., September 1, 2000; 51(350): 1543 - 1553.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Vissenberg, I. M. Martinez-Vilchez, J.-P. Verbelen, J. G. Miller, and S. C. Fry
In Vivo Colocalization of Xyloglucan Endotransglycosylase Activity and Its Donor Substrate in the Elongation Zone of Arabidopsis Roots
PLANT CELL, July 1, 2000; 12(7): 1229 - 1238.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
T. E. Proseus, J. K.E. Ortega, and J. S. Boyer
Separating Growth from Elastic Deformation during Cell Enlargement
Plant Physiology, February 1, 1999; 119(2): 775 - 784.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
C. Vargas-Rechia, F. Reicher, M. Rita Sierakowski, A. Heyraud, H. Driguez, and Y. Liénart
Xyloglucan Octasaccharide XXLGol Derived from the Seeds of Hymenaea courbaril Acts as a Signaling Molecule
Plant Physiology, March 1, 1998; 116(3): 1013 - 1021.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1993 by the American Society of Plant Biologists