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PLANT PHYSIOLOGY , Vol 103, Issue 4 1055-1059, Copyright © 1993 by American Society of Plant Biologists

MOLECULAR BIOLOGY AND GENE REGULATION

Recognition of Peroxisomes by Immunofluorescence in Transformed and Untransformed Tobacco Cells

J. L. Marrison, I. Onyeocha, A. Baker and R. M. Leech
Department of Biology, University of York, Heslington, York, YO1 5DD, United Kingdom (J.L.M., R.M.L.)

We report the visualization of peroxisomes in tobacco (Nicotiana tabacum) leaves using fluorescently labeled antibodies to glycolate oxidase. In transgenic tobacco leaves the expression of isocitrate lyase was also visualized. In dual probing experiments both enzymes were shown to be present together in all peroxisomes in transgenic tobacco leaves.


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K. Maxwell, J. L. Marrison, R. M. Leech, H. Griffiths, and P. Horton
Chloroplast Acclimation in Leaves of Guzmania monostachia in Response to High Light
Plant Physiology, September 1, 1999; 121(1): 89 - 96.
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Copyright © 1993 by the American Society of Plant Biologists