Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (30)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Karpinski, S.
Right arrow Articles by Hallgren, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karpinski, S.
Right arrow Articles by Hallgren, J. E.
Agricola
Right arrow Articles by Karpinski, S.
Right arrow Articles by Hallgren, J. E.

PLANT PHYSIOLOGY , Vol 103, Issue 4 1385-1391, Copyright © 1993 by American Society of Plant Biologists

MOLECULAR BIOLOGY AND GENE REGULATION

Molecular Responses to Photooxidative Stress in Pinus sylvestris (L.) (II. Differential Expression of CuZn-Superoxide Dismutases and Glutathione Reductase

S. Karpinski, G. Wingsle, B. Karpinska and J. E. Hallgren
Department of Forest Genetics and Plant Physiology, Faculty of Forestry, Swedish University of Agricultural Science, S-901 83 Umea, Sweden

The influence of photooxidative stress on genes expressing superoxide dismutase (Sod) and glutathione reductase (Gor) was analyzed in needles of top and side shoots of 3-year-old Pinus sylvestris (L.) seedlings. The study was carried out in the field during spring recovery. From mid-April the top shoots of seedlings protruded above the snow and thus were exposed to sunlight, whereas the side shoots were covered with snow until May 4. Needles were sampled from top and side shoots on five different occasions. At the beginning of May the mRNA levels for cytosolic CuZn-Sod were significantly higher in top-shoot needles than in side-shoot needles. Similar results were obtained for chloroplastic CuZn-Sod mRNA. After May 6 we could not detect any significant differences between top- and side-shoot needles for either CuZn-Sod mRNA level. Transcript accumulation for the chloroplastic CuZn-Sod was up to 4-fold higher than for cytosolic CuZn-Sod in both types of shoots. On June 1 minimum transcript levels were observed for both CuZn-SOD isoforms. Protein activity analysis for CuZn-SOD isozymes did not reveal any significant differences between top- and side-shoot needles during the whole period of measurements. The mRNA level for chloroplastic Gor was similar in both types of shoots. However, the total GR activity was significantly higher in top-shoot needles than in side-shoot needles at the beginning of May. The analysis of mRNA accumulation for chloroplastic CuZn-Sod and Gor indicates that transcript levels were at least 5- to 20-fold higher for CuZn-Sod than for chloroplastic Gor. The differential expressions of Sod and Gor genes are discussed in relation to regulation of the enzymic scavenging system during photooxidative stress conditions.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
C. C.C. Chang, I. Slesak, L. Jorda, A. Sotnikov, M. Melzer, Z. Miszalski, P. M. Mullineaux, J. E. Parker, B. Karpinska, and S. Karpinski
Arabidopsis Chloroplastic Glutathione Peroxidases Play a Role in Cross Talk between Photooxidative Stress and Immune Responses
Plant Physiology, June 1, 2009; 150(2): 670 - 683.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. R. Aluru, J. Zola, A. Foudree, and S. R. Rodermel
Chloroplast Photooxidation-Induced Transcriptome Reprogramming in Arabidopsis immutans White Leaf Sectors
Plant Physiology, June 1, 2009; 150(2): 904 - 923.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
I. Slesak, B. Karpinska, E. Surowka, Z. Miszalski, and S. Karpinski
Redox Changes in the Chloroplast and Hydrogen Peroxide are Essential for Regulation of C3-CAM Transition and Photooxidative Stress Responses in the Facultative CAM Plant Mesembryanthemum crystallinum L.
Plant Cell Physiol., June 15, 2003; 44(6): 573 - 581.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
S. Karpinski, H. Reynolds, B. Karpinska, G. Wingsle, G. Creissen, and P. Mullineaux
Systemic Signaling and Acclimation in Response to Excess Excitation Energy in Arabidopsis
Science, April 23, 1999; 284(5414): 654 - 657.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1993 by the American Society of Plant Biologists