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PLANT PHYSIOLOGY , Vol 104, Issue 2 497-503, Copyright © 1994 by American Society of Plant Biologists
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MOLECULAR BIOLOGY AND GENE REGULATION |
Expression of a Soybean (Glycine max [L.] Merr.) Seed Storage Protein Gene in Transgenic Arabidopsis thaliana and Its Response to Nutritional Stress and to Abscisic Acid Mutations
S. Naito, M. Y. Hirai, M. Chino and Y. Komeda
Molecular Genetics Research Laboratory, University of Tokyo, Hongo Tokyo 113, Japan (S.N., Y.K.)
Among the three subunits of [beta]-conglycinin, the 7S seed storage protein
of soybean (Glycine max [L.] Merr.), expression of the [beta] subunit gene
is unique. Accumulation of the [beta] subunit is enhanced in
sulfate-deficient soybean plants, and its mRNA levels increase when
abscisic acid (ABA) is added to the in vitro cotyledon culture medium.
Transgenic Arabidopsis thaliana lines carrying a gene encoding the [beta]
subunit was constructed and grown under sulfate deficiency. Accumulation of
both [beta] subunit mRNA and protein were enhanced in developing A.
thaliana seeds. Accumulation of one of the A. thaliana seed storage protein
mRNAs was also enhanced by sulfate deficiency, although the response was
weaker than that observed for the soybean [beta] subunit mRNA. When the
aba1-1 or abi3-1 mutations were crossed into the transgenic A. thaliana
line, accumulation of the [beta] subunit was significantly reduced, whereas
accumulation of the A. thaliana seed storage protein was not greatly
affected. These results indicate that soybean and A. thaliana share a
common mechanism for response to sulfate deficiency and to ABA, although
the sensitivity is different between the species. The transgenic A.
thaliana carrying the [beta] subunit gene of [beta]-conglycinin will be a
good system to analyze these responses.
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