Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (33)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, J. Z.
Right arrow Articles by Harada, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, J. Z.
Right arrow Articles by Harada, J. J.
Agricola
Right arrow Articles by Zhang, J. Z.
Right arrow Articles by Harada, J. J.

PLANT PHYSIOLOGY , Vol 104, Issue 3 857-864, Copyright © 1994 by American Society of Plant Biologists

MOLECULAR BIOLOGY AND GENE REGULATION

Isocitrate Lyase and Malate Synthase Genes from Brassica napus L. Are Active in Pollen

J. Z. Zhang, D. L. Laudencia-Chingcuanco, L. Comai, M. Li and J. J. Harada
Section of Plant Biology, Division of Biological Sciences, University of California, Davis, California 95616

To investigate if pollen possesses glyoxysomal function, we analyzed the activities of isocitrate lyase and malate synthase genes. Because the activities of these enzymes were exceedingly low in pollen extracts, we constructed fusion genes encoding [beta]-glucuronidase (GUS) that are regulated by isocitrate lyase or malate synthase promoters from Brassica napus L. to increase the sensitivity of our assays. Expression of the fusion genes in transgenic tobacco was qualitatively similar to that of the endogenous genes; GUS activity was low in dry seeds, maximal in seedlings, and very low or undetectable in leaves, indicating that the promoters are regulated correctly. We showed that isocitrate lyase and malate synthase genes are active at specific stages of pollen development and that their activities are not enhanced during pollen germination in transgenic tobacco. We also confirmed that the endogenous genes are active by showing that the corresponding mRNAs could be detected in pollen at specific stages of development. The activation of the isocitrate lyase and malate synthase genes suggests that glyoxysomal function is induced during pollen development.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. Footitt, D. Dietrich, A. Fait, A. R. Fernie, M. J. Holdsworth, A. Baker, and F. L. Theodoulou
The COMATOSE ATP-Binding Cassette Transporter Is Required for Full Fertility in Arabidopsis
Plant Physiology, July 1, 2007; 144(3): 1467 - 1480.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Joosen, J. Cordewener, E. D. J. Supena, O. Vorst, M. Lammers, C. Maliepaard, T. Zeilmaker, B. Miki, T. America, J. Custers, et al.
Combined Transcriptome and Proteome Analysis Identifies Pathways and Markers Associated with the Establishment of Rapeseed Microspore-Derived Embryo Development
Plant Physiology, May 1, 2007; 144(1): 155 - 172.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E.-C. Pua, S. Chandramouli, P. Han, and P. Liu
Malate synthase gene expression during fruit ripening of Cavendish banana (Musa acuminata cv. Williams)
J. Exp. Bot., January 2, 2003; 54(381): 309 - 316.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. J. Eastmond, V. Germain, P. R. Lange, J. H. Bryce, S. M. Smith, and I. A. Graham
Postgerminative growth and lipid catabolism in oilseeds lacking the glyoxylate cycle
PNAS, May 9, 2000; 97(10): 5669 - 5674.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1994 by the American Society of Plant Biologists