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PLANT PHYSIOLOGY , Vol 104, Issue 3 873-880, Copyright © 1994 by American Society of Plant Biologists
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MOLECULAR BIOLOGY AND GENE REGULATION |
Developmental and Environmental Regulation of the Nicotiana plumbaginifolia Cytosolic Cu/Zn-Superoxide Dismutase Promoter in Transgenic Tobacco
D. Herouart, M. Van Montagu and D. Inze
Laboratorium voor Genetica (D.H., M.V.M.) and Laboratoire Associe de I'Institut National de la Recherche Agronomique (France) (D.I.), Universiteit Gent, B-9000 Gent, Belgium
Superoxide dismutases (SODs) play a key role in the cellular defense
against reactive oxygen species. To study the transcriptional regulation at
the cellular level, the promoter of the Nicotiana plumbaginifolia cytosolic
gene encoding Cu/ZnSOD (SODCc) was fused to the [beta]-glucuronidase (GUS)
reporter gene (gusA) and analyzed in transgenic tobacco plants. The
promoter was highly active in vascular bundles of leaves and stems, where
it is confined to phloem cells. In flowers, GUS activity was detected in
ovules and pollen grains, in pigmented tissues of petals, and in vascular
tissue of ovaries and anthers. In response to treatment with the
superoxide-generating herbicide paraquat, very strong GUS stainng was
observed in photosynthetically active cells of leaves and in some epidermal
root cells of seedlings. The expression of the SODCc-gusA was also induced
in seedlings after heat shock and chilling and after treatment with
sulfhydryl antioxidants such as reduced glutathione and cysteine. It is
postulated that SODCc expression is directly linked to a cell-specific
production of excess superoxide radicals in the cytosol.
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