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PLANT PHYSIOLOGY , Vol 104, Issue 4 1131-1138, Copyright © 1994 by American Society of Plant Biologists
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ENVIRONMENTAL AND STRESS PHYSIOLOGY |
Low Temperature-Induced Cytoplasmic Acidosis in Cultured Mung Bean (Vigna radiata [L.] Wilczek) Cells
S. Yoshida
The Institute of Low Temperature Science, Hokkaido University, Sapporo, 060 Japan
Cold-induced changes in vivo in the cytoplasmic pH of suspension-cultured
cells of mung bean (Vigna radiata [L.] Wilczek) were investigated by
fluorescence-ratio imaging cryomicroscopy with special reference to the
variations in the chilling sensitivity of cells during the growth cycle.
Because of the preferential localization of the fluorophore in the
cytoplasm under specified conditions and the ideal response of fluorescence
to pH, fluorescein diacetate allows measurements to be made of temporal
changes in cytoplasmic pH at low temperature. A remarkable difference was
demonstrated in the cold-induced changes in cytoplasmic pH between cells at
the early and late stages of exponential growth. The cells at the early
stage of exponential growth were most sensitive to chilling, and the
cytoplasmic pH decreased dramatically within a short period of incubation
at 0[deg]C, decreasing from 7.4 to 6.8 after 4 h and to 6.3 after 18 h. The
cells at the late stage of exponential growth were chilling tolerant, and
no significant decrease in the cytoplasmic pH was observed during the
incubation at 0[deg]C for 24 h or even longer. From the results presented
here, it appears that cold-induced cytoplasmic acidosis is characteristic
of chilling-sensitive mung bean suspension-cultured cells.
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