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PLANT PHYSIOLOGY , Vol 104, Issue 4 1455-1458, Copyright © 1994 by American Society of Plant Biologists


RAPID COMMUNICATIONS

The Role of Ascorbate Free Radical as an Electron Acceptor to Cytochrome b-Mediated Trans-Plasma Membrane Electron Transport in Higher Plants

N. Horemans, H. Asard and R. J. Caubergs
Department of Biology, University of Antwerp (Universitair Centrum Antwerpen), Groenenborgerlaan 171, B-2020 Antwerp, Belgium

The action of ascorbate free radical as an electron acceptor to cytochrome b-mediated trans-plasma membrane electron transport is demonstrated. Addition of ascorbate free radical to ascorbate-loaded plasma membrane vesicles caused a rapid oxidation of the cytochrome, followed by a slower re-reduction. The fully reduced dehydroascorbate was ineffective.


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