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PLANT PHYSIOLOGY , Vol 105, Issue 1 133-139, Copyright © 1994 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

Identification of Intracellular Carbonic Anhydrase in Chlamydomonas reinhardtii with a Carbonic Anhydrase-Directed Photoaffinity Label

H. D. Husic and C. A. Marcus
Department of Chemistry, Lafayette College, Easton, Pennsylvania 18042-1782

A carbonic anhydrase (CA)-directed photoaffinity reagent, 125I-labeled p-aminomethylbenzenesulfonamide-4-azidosalicylamide,was synthesized and shown to derivatize periplasmic CA in the unicellular green alga Chlamydomonas reinhardtii. The photoderivatization of purified C. reinhardtii periplasmic CA or intact C. reinhardtii cells with the reagent resulted in the modification of the large (37 kD) subunit of the enzyme. Photoderivatization of proteins in lysed C. reinhardtii cells also resulted in the specific labeling of a polypeptide of 30 kD. Centrifugation of the cell extract prior to photoaffinity labeling revealed that the labeled peptide was present predominantly in a particulate fraction. The photoaffinity-labeled 30-kD polypeptide was not observed in extracts from a mutant of C. reinhardtii that is believed to be deficient in an intracellular form of CA. These results provide evidence that the 30-kD polypeptide, which is photoaffinity labeled in lysed C. reinhardtii cells, is an intracellular form of CA.


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D. T. Hanson, L. A. Franklin, G. Samuelsson, and M. R. Badger
The Chlamydomonas reinhardtii cia3 Mutant Lacking a Thylakoid Lumen-Localized Carbonic Anhydrase Is Limited by CO2 Supply to Rubisco and Not Photosystem II Function in Vivo
Plant Physiology, August 1, 2003; 132(4): 2267 - 2275.
[Abstract] [Full Text] [PDF]

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S. Mitsuhashi and S. Miyachi
Amino Acid Sequence Homology between N- and C-terminal Halves of a Carbonic Anhydrase in Porphyridium purpureum, as Deduced from the Cloned cDNA
J. Biol. Chem., November 8, 1996; 271(45): 28703 - 28709.
[Abstract] [Full Text] [PDF]


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Copyright © 1994 by the American Society of Plant Biologists