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PLANT PHYSIOLOGY , Vol 105, Issue 1 199-203, Copyright © 1994 by American Society of Plant Biologists
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METABOLISM AND ENZYMOLOGY |
Pectin Methylesterase Isoforms in Tomato (Lycopersicon esculentum) Tissues (Effects of Expression of a Pectin Methylesterase Antisense Gene)
J. Gaffe, D. M. Tieman and A. K. Handa
Horticulture Department, Purdue University, 1165 Horticulture Building, West Lafayette, Indiana 47907-1165
We have identified two major groups of pectin methylesterase (PME, EC
3.1.1.11) isoforms in various tissues of tomatoes (Lycopersicon
esculentum). These two groups exhibited differential
immuno-cross-reactivity with polyclonal antibodies raised against tomato
fruit PME or flax callus PME and differences in their accumulation patterns
in tissues of wild-type and transgenic tomato plants expressing a PME
antisense gene. The group I isoforms with isoelectric points (pls) of 8.2,
8.4, and 8.5 are specific to fruit tissue, where they are the major forms
of PME activity. The group II PME isoforms, with pl values of 9 and above,
are observed in both vegetative and fruit tissues. The group I isoforms
cross-react with polyclonal antibodies raised to a PME isoform purified
from fruit, whereas the group II isoforms cross-react with antibodies to a
PME purified from flax callus. Expression of a fruit-specific PME
anti-sense gene impairs accumulation of the group I PME isoforms, with no
apparent effect on the accumulation of the group II PME isoforms. The
absence of any noticeable effects on growth and development of transgenic
plants suggests that the group I PME isoforms are not involved in plant
growth and development and may play a role under special circumstances such
as cell separation during fruit ripening.
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