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PLANT PHYSIOLOGY , Vol 105, Issue 2 491-496, Copyright © 1994 by American Society of Plant Biologists
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MOLECULAR BIOLOGY AND GENE REGULATION |
Complementation of the Tomato anthocyanin without (aw) Mutant Using the Dihydroflavonol 4-Reductase Gene
A. Goldsbrough, F. Belzile and J. I. Yoder
Department of Vegetable Crops, University of California, Davis, Davis, California 95616
We isolated the dihydroflavonol 4-reductase (DFR) gene from tomato
(Lycopersicon esculentum) using a previously characterized cDNA as probe.
Earlier studies had indicated that the DFR gene is present in tomato as a
single gene located on chromosome 2 near the locus anthocyanin without
(aw). Mutant alleles of the aw locus result in the complete absence of
anthocyanin pigmentation throughout all stages of plant development. When
the genomic DFR clone was introduced by Agrobacterium-mediated
transformation into plants bearing the aw mutation, primary transgenic
seedlings accumulated anthocyanins that could be observed while the plants
were still in tissue culture and which continued to be observed as the
plants matured. Progeny of self pollinated and backcrossed transgenic
plants segregated for anthocyanin pigmentation, and Southern hybridization
analyses indicated the presence of the DFR transgene exclusively in those
plants with pigmentation. These data indicate that the aw locus likely
corresponds to the structural gene for DFR and that DFR can be used as a
visual, nondestructive, plant-derived marker gene for tomato.
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