Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (40)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Galvez, S.
Right arrow Articles by Gadal, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Galvez, S.
Right arrow Articles by Gadal, P.
Agricola
Right arrow Articles by Galvez, S.
Right arrow Articles by Gadal, P.

PLANT PHYSIOLOGY , Vol 105, Issue 2 593-600, Copyright © 1994 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

Purification and Characterization of Chloroplastic NADP-Isocitrate Dehydrogenase from Mixotrophic Tobacco Cells (Comparison with the Cytosolic Isoenzyme)

S. Galvez, E. Bismuth, C. Sarda and P. Gadal
Physiologie Vegetale Moleculaire, Unite de Recherche Associee au Centre National de la Recherche Scientifique 1128, Bat 430, Universite Paris-Sud, 91405-Orsay Cedex, France

Green, mixotrophic tobacco (Nicotiana tabacum) cell cultures in the exponential growth phase were found to have two clearly distinguishable NADP-isocitrate dehydrogenase (ICDH; EC 1.1.1.42) isoenzymes. Their elution behavior during anion-exchange column chromatography was similar to that described previously for the cytosolic (ICDH1) and chloroplastic (ICDH2) enzymes from pea (Pisum sativum) leaves. ICDH2 was absent in etiolated tobacco cell suspensions and appeared during the greening process. Both isoforms were purified to apparent electrophoretic homogeneity by ammonium sulfate fractionation and anion-exchange and affinity chromatography. The isoenzymes were separated on a DEAE-Sephacel column, but the most effective step was a Matrex Red-A column, which enabled an overall purification of 833- and 1328-fold for ICDH1 and ICDH2, respectively. Polyclonal antibodies were raised against each isoform. The ICDH2-specific antibody was used to localize tobacco leaf ICDH2 in situ by an immunogold labeling technique. The enzyme was found largely, if not exclusively, in the chloroplasts of green leaves. ICDH1 and ICDH2 were shown to have apparent native molecular weights of 117,000 and 136,000, respectively, and to consist of identical, 48.5-kD subunits. Similar apparent Km values for NADP, D(+)isocitrate, and Mg2+ were found for the two enzymes when assayed with Mg2+ as the metal cofactor.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Sienkiewicz-Porzucek, A. Nunes-Nesi, R. Sulpice, J. Lisec, D. C. Centeno, P. Carillo, A. Leisse, E. Urbanczyk-Wochniak, and A. R. Fernie
Mild Reductions in Mitochondrial Citrate Synthase Activity Result in a Compromised Nitrate Assimilation and Reduced Leaf Pigmentation But Have No Effect on Photosynthetic Performance or Growth
Plant Physiology, May 1, 2008; 147(1): 115 - 127.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Lemaitre, E. Urbanczyk-Wochniak, V. Flesch, E. Bismuth, A. R. Fernie, and M. Hodges
NAD-Dependent Isocitrate Dehydrogenase Mutants of Arabidopsis Suggest the Enzyme Is Not Limiting for Nitrogen Assimilation
Plant Physiology, July 1, 2007; 144(3): 1546 - 1558.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
D. S. Skopelitis, N. V. Paranychianakis, K. A. Paschalidis, E. D. Pliakonis, I. D. Delis, D. I. Yakoumakis, A. Kouvarakis, A. K. Papadakis, E. G. Stephanou, and K. A. Roubelakis-Angelakis
Abiotic Stress Generates ROS That Signal Expression of Anionic Glutamate Dehydrogenases to Form Glutamate for Proline Synthesis in Tobacco and Grapevine
PLANT CELL, October 1, 2006; 18(10): 2767 - 2781.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Abiko, M. Obara, A. Ushioda, T. Hayakawa, M. Hodges, and T. Yamaya
Localization of NAD-Isocitrate Dehydrogenase and Glutamate Dehydrogenase in Rice Roots: Candidates for Providing Carbon Skeletons to NADH-Glutamate Synthase
Plant Cell Physiol., October 1, 2005; 46(10): 1724 - 1734.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Dutilleul, C. Lelarge, J.-L. Prioul, R. De Paepe, C. H. Foyer, and G. Noctor
Mitochondria-Driven Changes in Leaf NAD Status Exert a Crucial Influence on the Control of Nitrate Assimilation and the Integration of Carbon and Nitrogen Metabolism
Plant Physiology, September 1, 2005; 139(1): 64 - 78.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
C. Chaffei, K. Pageau, A. Suzuki, H. Gouia, M. H. Ghorbel, and C. Masclaux-Daubresse
Cadmium Toxicity Induced Changes in Nitrogen Management in Lycopersicon esculentum Leading to a Metabolic Safeguard Through an Amino Acid Storage Strategy
Plant Cell Physiol., November 15, 2004; 45(11): 1681 - 1693.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. R. Gray, A. R. Villarimo, C. L. Whitehead, and L. McIntosh
Transgenic Tobacco (Nicotiana tabacum L.) Plants with Increased Expression Levels of Mitochondrial NADP+-dependent Isocitrate Dehydrogenase: Evidence Implicating this Enzyme in the Redox Activation of the Alternative Oxidase
Plant Cell Physiol., October 15, 2004; 45(10): 1413 - 1425.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Fukao, M. Hayashi, I. Hara-Nishimura, and M. Nishimura
Novel Glyoxysomal Protein Kinase, GPK1, Identified by Proteomic Analysis of Glyoxysomes in Etiolated Cotyledons of Arabidopsis thaliana
Plant Cell Physiol., October 15, 2003; 44(10): 1002 - 1012.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Hodges
Enzyme redundancy and the importance of 2-oxoglutarate in plant ammonium assimilation
J. Exp. Bot., April 15, 2002; 53(370): 905 - 916.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. J. Corpas, J. B. Barroso, L. M. Sandalio, J. M. Palma, J. A. Lupiáñez, and L. A. del Río
Peroxisomal NADP-Dependent Isocitrate Dehydrogenase. Characterization and Activity Regulation during Natural Senescence
Plant Physiology, November 1, 1999; 121(3): 921 - 928.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
M. Lancien, S. Ferrario-Méry, Y. Roux, E. Bismuth, C. Masclaux, B. Hirel, P. Gadal, and M. Hodges
Simultaneous Expression of NAD-Dependent Isocitrate Dehydrogenase and Other Krebs Cycle Genes after Nitrate Resupply to Short-Term Nitrogen-Starved Tobacco
Plant Physiology, July 1, 1999; 120(3): 717 - 726.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
J. Palomo, F. Gallardo, M. F. Suárez, and F. M. Cánovas
Purification and Characterization of NADP+-Linked Isocitrate Dehydrogenase from Scots Pine . Evidence for Different Physiological Roles of the Enzyme in Primary Development
Plant Physiology, October 1, 1998; 118(2): 617 - 626.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
J. M. Martínez-Rivas and J. M. Vega
Purification and Characterization of NAD-Isocitrate Dehydrogenase from Chlamydomonas reinhardtii
Plant Physiology, September 1, 1998; 118(1): 249 - 255.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
V. Boiffin, M. Hodges, S. Gálvez, R. Balestrini, P. Bonfante, P. Gadal, and F. Martin
Eucalypt NADP-Dependent Isocitrate Dehydrogenase . cDNA Cloning and Expression in Ectomycorrhizae
Plant Physiology, July 1, 1998; 117(3): 939 - 948.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Galvez, O. Roche, E. Bismuth, S. Brown, P. Gadal, and M. Hodges
Mitochondrial localization of a NADR-dependent isocitrate dehydrogenase isoenzyme by using the green fluorescent protein as a marker
PNAS, June 23, 1998; 95(13): 7813 - 7818.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Szewczyk, A. Andrianopoulos, M. A. Davis, and M. J. Hynes
A Single Gene Produces Mitochondrial, Cytoplasmic, and Peroxisomal NADP-dependent Isocitrate Dehydrogenase in Aspergillus nidulans
J. Biol. Chem., September 28, 2001; 276(40): 37722 - 37729.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1994 by the American Society of Plant Biologists