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PLANT PHYSIOLOGY , Vol 105, Issue 2 601-605, Copyright © 1994 by American Society of Plant Biologists
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ENVIRONMENTAL AND STRESS PHYSIOLOGY |
Genetic Enhancement of Cold Tolerance by Expression of a Gene for Chloroplast [omega]-3 Fatty Acid Desaturase in Transgenic Tobacco
H. Kodama, T. Hamada, G. Horiguchi, M. Nishimura and K. Iba
Department of Biology, Faculty of Science, Kyushu University 33, Fukuoka 812, Japan
The increased production of trienoic fatty acids, hexadecatrienoic (16:3)
and linolenic (18:3) acids, is a response connected with cold acclimation
of higher plants and is thought to protect plant cells against cold damage.
Transgenic tobacco (Nicotiana tabacum cv SR1) plants that contain increased
levels of 16:3 and 18:3 fatty acids, and correspondingly decreased levels
of their precursors, hexadecadienoic and linoleic acids, were engineered by
introduction of a chloroplast [omega]-3 fatty acid desaturase gene (the
fad7 gene) isolated from Arabidopsis thaliana. When exposed to 1[deg]C for
7 d and then cultured at 25[deg]C, the suppression of leaf growth observed
in the wild-type plants was significantly alleviated in the transgenic
plants with the fad7 gene. The low-temperature- induced chlorosis was also
much reduced in the plants transformed with the fad7 gene. These results
indicate that increased levels of trienoic fatty acids in genetically
engineered plants enhance cold tolerance.
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