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PLANT PHYSIOLOGY , Vol 105, Issue 2 611-617, Copyright © 1994 by American Society of Plant Biologists
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MOLECULAR BIOLOGY AND GENE REGULATION |
Structure and Expression of an Arabidopsis Acetyl-Coenzyme A Carboxylase Gene
K. R. Roesler, B. S. Shorrosh and J. B. Ohlrogge
Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824
Acetyl-coenzyme A carboxylase (ACCase) catalyzes the formation of
malonyl-coenzyme A, which is used in the plastid for fatty acid synthesis
and in the cytosol for several pathways including fatty acid elongation and
flavonoid synthesis. Two overlapping Arabidopsis genomic clones were
isolated and sequenced to determine the entire ACCase-coding region. Thirty
introns with an average size of 94 bp were identified by comparison with an
alfalfa ACCase cDNA sequence. The 10-kb Arabidopsis ACCase gene encodes a
251-kD polypeptide, which has 80% amino acid sequence identity with alfalfa
ACCase and about 40% identity with ACCase of rat, chicken, yeast, and the
diatom Cyclotella. No chloroplast transit peptide sequence was observed,
suggesting that this Arabidopsis gene encodes a cytosolic ACCase isozyme.
ACCase gene transcripts were detected by RNase protection assays in
Arabidopsis root, leaf, silique, and seed. Genomic DNA blot analysis
revealed the presence of a second related Arabidopsis ACCase gene.
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