PLANT PHYSIOLOGY , Vol 105, Issue 4 1217-1222, Copyright © 1994 by American Society of Plant Biologists
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ENVIRONMENTAL AND STRESS PHYSIOLOGY |
Expression of Early Light-Inducible Proteins in Flag Leaves of Field-Grown Barley
K. Humbeck, K. Kloppstech and K. Krupinska
Institut fur Allgemeine Botanik, Universitat Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany (K.H., K.Krupinska)
Early light-inducible protein (ELIP) mRNA and protein levels were analyzed
during maturation and senescence of barley (Hordeum vulgare L.) flag leaves
under field conditions. The data clearly demonstrate that ELIP mRNA levels
are related to the sunlight intensity before sample collection. Levels of
mRNAs encoding both low and high molecular mass ELIPs fluctuate in
parallel. Changes in mRNA levels are accompanied by corresponding changes
in protein levels except for days when average temperatures are high.
Comparison of flag leaves at different stages of development in spring and
winter barley varieties suggests that light-stress-regulated ELIP gene
expression is independent of the developmental stage of the leaves.
Although chlorophyll content, photosystem II (PSII) efficiency, and 32-kD
herbicide-binding protein of PSII levels decrease drastically after the
onset of senescence, ELIP mRNA and protein still accumulate to high levels
on bright days.
