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PLANT PHYSIOLOGY , Vol 105, Issue 4 1355-1364, Copyright © 1994 by American Society of Plant Biologists
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MOLECULAR BIOLOGY AND GENE REGULATION |
The Role of Plastids in the Expression of Nuclear Genes for Thylakoid Proteins Studied with Chimeric [beta]-Glucuronidase Gene Fusions
C. Bolle, S. Sopory, T. Lubberstedt, R. B. Klosgen, R. G. Herrmann and R. Oelmuller
Botanisches Institut der Ludwig-Maximilians-Universitat, Menzingerstrasse 67, 80638 Munchen, Germany
We have analyzed plastid and nuclear gene expression in tobacco seedlings
using the carotenoid biosynthesis inhibitor nor-flurazon. mRNA levels for
three nuclear-encoded chlorophyll-binding proteins of photosystem I and
photosystem II (CAB I and II and the CP 24 apoprotein) are no longer
detectable in photobleached seedlings, whereas those for other components
of the thylakoid membrane (the 33- and 23-kD polypeptides and Rieske Fe/S
polypeptide) accumulate to some extent. Transgenic tobacco seedlings with
promoter fusions from genes for thylakoid membrane proteins exhibit a
similar expression behavior: a CAB-[beta]-glucuronidase (GUS) gene fusion
is not expressed in herbicide-treated seedlings, whereas PC-, FNR-, PSAF-,
and ATPC-promoter fusions are expressed, although at reduced levels. All
identified segments in nuclear promoters analyzed that have been shown to
respond to light also respond to photodamage to the plastids. Thus, the
regulatory signal pathways either merge prior to gene regulation or
interact with closely neighboring cis elements. These results indicate that
plastids control nuclear gene expression via different and gene-specific
cis-regulatory elements and that CAB gene expression is different from the
expression of the other genes tested. Finally, a plastid-directing import
sequence from the maize Waxy gene is capable of directing the GUS protein
into the photodamaged organelle. Therefore, plastid import seems to be
functional in photobleached organelles.
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