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PLANT PHYSIOLOGY , Vol 106, Issue 1 17-23, Copyright © 1994 by American Society of Plant Biologists
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MOLECULAR BIOLOGY AND GENE REGULATION |
Herbicide-Resistant Tobacco Plants Expressing the Fused Enzyme between Rat Cytochrome P4501A1 (CYP1A1) and Yeast NADPH-Cytochrome P450 Oxidoreductase
N. Shiota, A. Nagasawa, T. Sakaki, Y. Yabusaki and H. Ohkawa
The Graduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657, Japan (N.S., H.O.)
Transgenic tobacco (Nicotiana tabacum cv Xanthi) plants expressing a
genetically engineered fused enzyme between rat cytochrome P4501A1 (CYP1A1)
and yeast NADPH-cytochrome P450 oxidoreductase were produced. The
expression plasmid pGFC2 for the fused enzyme was constructed by insertion
of the corresponding cDNA into the expression vector pNG01 under the
control of the cauliflower mosaic virus 35S promoter and nopaline synthase
gene terminator. The fused enzyme cDNA was integrated into tobacco genomes
by Agrobacterium infection techniques. In transgenic tobacco plants, the
fused enzyme protein was localized primarily in the microsomal fraction.
The microsomal monooxygenase activities were approximately 10 times higher
toward both 7-ethoxycoumarin and benzo[a]pyrene than in the control plant.
The transgenic plants also showed resistance to the herbicide chlortoluron.
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