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PLANT PHYSIOLOGY , Vol 106, Issue 2 421-427, Copyright © 1994 by American Society of Plant Biologists
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ENVIRONMENTAL AND STRESS PHYSIOLOGY |
High-Oleate Oilseeds Fail to Develop at Low Temperature
M. F. Miquel and J. A. Browse
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340
The fad2 mutants of Arabidopsis thaliana are deficient in activity of the
endoplasmic reticulum oleate desaturase that is the main enzyme responsible
for polyunsaturated lipid synthesis in developing seeds of oil crops. A
comparison of wild-type and fad2 seeds developing on heterozygous (FAD2/-)
plants was used as a model for genetically engineered high-oleate oilseeds
of species such as soybean and canola. When fad2 seeds developed at normal
temperatures (22[deg]C), they showed high viability compared to wild-type
seeds. When a portion of seed development took place at 6[deg]C,
germination of the wild-type siblings remained high but germination of fad2
segregants declined considerably. This was true even when exposure to low
temperature was limited to the final stages of seed filling and maturation.
Compared to wild-type seeds, fully viable fad2 seeds produced at 22[deg]C
had reduced lipid contents and were slower to germinate at 10 and 6[deg]C.
Taken together, these results indicate that for some oilseed species at
least, molecular genetic manipulation of oleate levels in the oil may
result in plant lines with unacceptable performance in the field.
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